Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats.
collagen
osteoclast
periodontal therapy
photochemotherapy
Journal
Journal of periodontal research
ISSN: 1600-0765
Titre abrégé: J Periodontal Res
Pays: United States
ID NLM: 0055107
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
06
02
2020
revised:
15
04
2020
accepted:
21
05
2020
pubmed:
11
7
2020
medline:
20
1
2021
entrez:
11
7
2020
Statut:
ppublish
Résumé
The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research. Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar. After 7 days, ligature was removed and animals were treated according to the following experimental groups (n = 8): control-SRP plus saline solution; ALN-SRP plus ALN; aPDT-SRP plus methylene blue irrigation, followed by low-level laser therapy (LLLT); and ALN/aPDT-SRP plus ALN and methylene blue irrigation followed by LLLT. The animals were euthanized at 7, 15, and 30 days after treatments. Collagen maturation (picrosirius red staining) and immunohistochemical analyses (TRAP, RANKL and osteoprotegerin [OPG]) were performed. Data were submitted to statistical analysis (P < .05). At 7 days, group ALN presented a significantly higher number of TRAP-positive cells and percentage of immature collagen fibers than group ALN/aPDT, while group ALN/aPDT presented a significantly higher percentage of mature collagen fibers than group ALN. At 30 days, group ALN presented significantly lower percentage of immature collagen fibers and higher percentage of mature collagen fibers than control. It can be concluded that topical use of ALN coadjutant to SRP, alone or combined with aPDT, enhanced collagen maturation and reduced osteoclastogenesis during the healing of experimental periodontitis.
Sections du résumé
OBJECTIVE
OBJECTIVE
The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats.
BACKGROUND
BACKGROUND
Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research.
METHODS
METHODS
Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar. After 7 days, ligature was removed and animals were treated according to the following experimental groups (n = 8): control-SRP plus saline solution; ALN-SRP plus ALN; aPDT-SRP plus methylene blue irrigation, followed by low-level laser therapy (LLLT); and ALN/aPDT-SRP plus ALN and methylene blue irrigation followed by LLLT. The animals were euthanized at 7, 15, and 30 days after treatments. Collagen maturation (picrosirius red staining) and immunohistochemical analyses (TRAP, RANKL and osteoprotegerin [OPG]) were performed. Data were submitted to statistical analysis (P < .05).
RESULTS
RESULTS
At 7 days, group ALN presented a significantly higher number of TRAP-positive cells and percentage of immature collagen fibers than group ALN/aPDT, while group ALN/aPDT presented a significantly higher percentage of mature collagen fibers than group ALN. At 30 days, group ALN presented significantly lower percentage of immature collagen fibers and higher percentage of mature collagen fibers than control.
CONCLUSION
CONCLUSIONS
It can be concluded that topical use of ALN coadjutant to SRP, alone or combined with aPDT, enhanced collagen maturation and reduced osteoclastogenesis during the healing of experimental periodontitis.
Substances chimiques
Sodium
9NEZ333N27
Alendronate
X1J18R4W8P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
850-858Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2016/04163-0
Organisme : University of Western Sao Paulo
ID : #3080
Organisme : University of Western Sao Paulo
ID : #3771
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Graduate Program
Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Dutra BC, Oliveira AMSD, Oliveira PAD, et al. Effect of 1% sodium alendronate in the non-surgical treatment of periodontal intraosseous defects: a 6-month clinical trial. J Appl Oral Sci. 2017;25:307-310.
Oktay S, Chukkapalli SS, Rivera-Kweh MF, Velsko IM, Holliday LS, Kesavalu L. Periodontitis in rats induces systemic oxidative stress that is controlled by bone-targeted antiresorptives. J Periodontol. 2015;86:137-145.
Teles RP, Haffajee AD, Socransky SS. Microbiological goals of periodontal therapy. Periodontol. 2000;2006(42):180-218.
Kaldahl WB, Kalkwarf KL, Patil KD. A review of longitudinal studies that compared periodontal therapies. J Periodontol. 1993;64:243-253.
Fernandes LA, Martins TM, de Almeida JM, Theodoro LH, Garcia VG. Radiographic assessment of photodynamic therapy as an adjunctive treatment on induced periodontitis in immunosuppressed rats. J Appl Oral Sci. 2010;18:237-243.
Salvi GE, Lang NP. Host response modulation in the management of periodontal diseases. J Clin Periodontol. 2005;32:108-129.
Reddy MS, Geurs NC, Gunsolley JC. Periodontal host modulation with antiproteinase, anti-inflammatory, and bone-sparing agents. A systematic review . Ann Periodontol. 2003;8:12-37.
Russel RG. Bisphosphonates: the first 40 years. Bone. 2011;49:2-19.
De Colli M, Tortorella P, Agamennone M, et al. Bisphosfonate matrix metalloproteinase inhibitors for the treatment of periodontitis: an in vitro study. Int J Mol Med. 2018;42:651-657.
Im GI, Qureshi SA, Kenney J, Rubash HE, Shanbhag AS. Osteoblast proliferation and maturation by bisphosphonates. Biomaterials. 2004;25:4105-4115.
Akram Z, Abduljabbar T, Kellesarian SV, Abu Hassan MI, Javed F, Vohra F. Efficacy of bisphosphonate as an adjunct to nonsurgical periodontal therapy in the management of periodontal disease: a systematic review. Br J Clin Pharmacol. 2017;83:444-454.
Wanikar I, Rathod S, Kolte AP. Clinico-radiographic evaluation of 1% alendronate gel as an adjunct and smart blood derivative platelet rich fibrin in grade II furcation defects. J Periodontol. 2019;90:52-60.
De Almeida J, Ervolino E, Bonfietti LH, et al. Adjuvant therapy with sodium alendronate for the treatment of experimental periodontitis in rats. J Periodontol. 2015;86:1166-1175.
Pradeep A, Kumari M, Rao NS, Naik SB. 1% alendronate gel as local drug delivery in the treatment of class II furcation defects: a randomized controlled clinical trial. J Periodontol. 2013;84:307-315.
Sharma A, Pradeep A. Clinical efficacy of 1% alendronate gel as a local drug delivery system in the treatment of chronic periodontitis: a randomized, controlled clinical trial. J Periodontol. 2012;83:11-18.
Sharma A, Pradeep A. Clinical efficacy of 1% alendronate gel in adjunct to mechanotherapy in the treatment of aggressive periodontitis: a randomized controlled clinical trial. J Periodontol. 2012;83:19-26.
Pradeep A, Sharma A, Rao NS, Bajaj P, Naik SB, Kumari M. Local drug delivery of alendronate gel for the treatment of patients with chronic periodontitis with diabetes mellitus: a double-masked controlled clinical trial. J Periodontol. 2012;83:1322-1328.
Banfi S, Caruso E, Buccafurni L, et al. Antibacterial activity of tetraaryl-porphyrin photosensitizers: an in vitro study on Gram negative and Gram positive bacteria. J Photochem Photobiol B. 2006;85:28-38.
Kikuchi T, Mogi M, Okabe I, et al. Adjunctive application of antimicrobial photodynamic therapy in nonsurgical periodontal treatment: a review of literature. Int J Mol Sci. 2015;16:24111-24126.
Garcia VG, Gualberto Júnior EC, Ervolino E, Nagata MJH, De Almeida JM, Theodoro LH. aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine. Photodiagnosis Photodyn Ther. 2018;22:70-78.
Garcia VG, Longo M, Fernandes LA, et al. Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy. Lasers Med Sci. 2013;28:143-150.
Montes GS, Junqueira LCU. The use of the Picrosirius-polarization method for the study of the biopathology of collagen. Mem Inst Oswaldo Cruz. 1991;86:1-11.
Guimaraes-Stabili MR, De Aquino SG, De Almeida CF, et al. Systemic administration of curcumin or piperine enhances the periodontal repair: a preliminary study in rats. Clin Oral Investig. 2019;23:3297-3306.
Boyce BF, Xing L. Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys. 2008;473:139-146.
Souza PP, Lerner UH. The role of cytokines in inflammatory bone loss. Immunol Invest. 2013;42:555-622.
Harada S, Takahashi N. Control of bone resorption by RANKL-RANK system. Clin Calcium. 2011;21:1121-1130.
Takahashi N, Maeda K, Ishihara A, Uehara S, Kobayashi Y. Regulatory mechanism of osteoclastogenesis by RANKL and Wnt signals. Front Biosci (Landmark Ed). 2011;16:21-30.
Genaro V. Influência do alendronato sódico, da terapia fotodinâmica ou da associação de ambos como terapias adjuntas à raspagem e alisamento radicular no tratamento da periodontite experimental induzida. Estudo histoquímico em ratos. Presidente Prudente, SP: University of Western Sao Paulo. 2019;61pp. Dissertation.
Levi YLAS, Novais GS, Dias RB, et al. Effects of the prebiotic mannan oligosaccharide on the experimental periodontitis in rats. J Clin Periodontol. 2018;45:1078-1089.
Johnson IH. Effects of local irritation and dextran sulphate administration on the periodontium of the rat. J Periodontal Res. 1975;10:332-345.
Akyol UK, Sipal S, Demirci E, Gungormus M. The influence of low-level laser therapy with alendronate irrigation on healing of bone defects in rats. Lasers Med Sci. 2015;30:1141-1146.
Gajendrareddy PK, Junges R, Cygan G, Zhao Y, Marucha PT, Engeland CG. Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis. J Periodontal Res. 2017;52:644-649.
De Oliveira PG, Silveira e Souza AMM, Novaes AB, et al. Adjunctive effect of antimicrobial photodynamic therapy in induced periodontal disease. Animal study with histomorphometrical, immunohistochemical, and cytokine evaluation. Lasers Med Sci. 2016;31:1275-1283.
Van der Weijden GA, Timmerman MF. A systematic review on the clinical efficacy of subgingival debridement in the treatment of chronic periodontitis. J Clin Periodontol. 2002;29:55-71.
Slots J, Rams TE. Antibiotics in periodontal therapy: advantages and disadvantages. J Clin Periodontol. 1990;17:479-493.
Taubman MA, Kawai T, Han X. The new concept of periodontal disease pathogenesis requires new and novel therapeutic strategies. J Clin Periodontol. 2007;34:367-369.
Garcia VG, Longo M, Gualberto Júnior EC, et al. Effect of the concentration of phenothiazine photosensitizers in antimicrobial photodynamic therapy on bone loss and the immune inflammatory response of induced periodontitis in rats. J Periodontal Res. 2014;49:584-594.
De Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Bonfante S, Garcia VG. Treatment of experimental periodontal disease by photodynamic therapy in rats with diabetes. J Periodontol. 2008;79:2156-2165.
De Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Oshiiwa M, Garcia VG. In vivo effect of photodynamic therapy on periodontal bone loss in dental furcations. J Periodontol. 2008;79:1081-1088.
Chan Y, Lai CH. Bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. Lasers Med Sci. 2003;18:51-55.
Ren C, McGrath C, Jin L, Zhang C, Yang Y. The effectiveness of low-level laser therapy as an adjunct to non-surgical periodontal treatment: a meta-analysis. J Periodontal Res. 2017;52:8-20.
Gupta A, Dai T, Hamblin MR. Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice. Lasers Med Sci. 2014;29:257-265.
Vrahnas C, Buenzli PR, Pearson TA, et al. Differing effects of parathyroid hormone, alendronate, and odanacatib on bone formation and on the mineralization process in intracortical and endocortical bone of ovariectomized rabbits. Calcif Tissue Int. 2018;103:625-637.
Byrjalsen I, Leeming DJ, Qvist P, Christiansen C, Karsdal MA. Bone turnover and bone collagen maturation in osteoporosis: effects of antiresorptive therapies. Osteoporos Int. 2008;19:339-348.
Garnero P, Bauer DC, Mareau E, et al. Effects of PTH and alendronate on type I collagen isomerization in postmenopausal women with osteoporosis: the PaTH study. J Bone Miner Res. 2008;23:1442-1448.
Chen K, Qiu P, Yuan Y, et al. Pseurotin A inhibits osteoclastogenesis and prevents ovariectomized-induced bone loss by suppressing reactive oxygen species. Theranostics. 2019;9:1634-1650.
Li M, Chen X, Yan J, et al. Inhibition of osteoclastogenesis by stem cell-derived extracellular matrix through modulation of intracellular reactive oxygen species. Acta Biomater. 2018;71:118-131.
Camacho-Alonso F, Davia-Peña RS, Vilaplana-Vivo C, Tudela-Mulero MR, Merino JJ, Martínez-Beneyto Y. Synergistic effect of photodynamic therapy and alendronate on alveolar bone loss in rats with ligature-induced periodontitis. J Periodontal Res. 2018;53:306-314.
Ipshita S, Kurian IG, Dileep P, Kumar S, Singh P, Pradeep AR. One percent alendronate and aloe vera gel local host modulating agents in chronic periodontitis patients with class II furcation defects: A randomized, controlled clinical trial. J Investig Clin Dent. 2018;9:1-7.