Ruminal epithelial insulin-like growth factor-binding proteins 2, 3, and 6 are associated with epithelial cell proliferation.
Animals
Cattle
Cell Proliferation
/ drug effects
Cells, Cultured
Epithelial Cells
/ physiology
Fatty Acids, Volatile
/ pharmacology
Gene Expression
/ drug effects
Gene Expression Regulation, Developmental
/ genetics
Hydrogen-Ion Concentration
Insulin-Like Growth Factor Binding Protein 2
/ genetics
Insulin-Like Growth Factor Binding Protein 3
/ genetics
Insulin-Like Growth Factor Binding Protein 6
/ genetics
Insulin-Like Growth Factor I
/ metabolism
RNA, Messenger
/ genetics
Rumen
/ cytology
insulin-like growth factor-binding protein
rumen epithelial cell
short-chain fatty acid
Journal
Animal science journal = Nihon chikusan Gakkaiho
ISSN: 1740-0929
Titre abrégé: Anim Sci J
Pays: Australia
ID NLM: 100956805
Informations de publication
Date de publication:
Historique:
received:
09
03
2020
revised:
02
06
2020
accepted:
10
06
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
31
10
2020
Statut:
ppublish
Résumé
The aim of this study was to identify factors that regulate ruminal epithelial insulin-like growth factor-binding protein (IGFBP) expression and determine its role in rumen epithelial cell proliferation. Primary bovine rumen epithelial cells (BREC) were incubated with short-chain fatty acids (SCFAs) at pH 7.4 or 5.6, lactate, lipopolysaccharide (LPS), insulin-like growth factor-I (IGF-I), -II (IGF-II), or recombinant bovine IGFBP2 (rbIGFBP2). The mRNA expression levels of IGFBP in BREC were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation rate of BREC was analyzed using a WST-1 assay. IGFBP2 gene expression tended to be lower with SCFA treatment (p < .1), and IGFBP6 gene expression was significantly lower with SCFA treatment (p < .05). IGFBP3 and IGFBP6 gene expression tended to be higher with d-Lactate treatment (p < .1). IGFBP3 gene expression was significantly higher (p < .05) with LPS treatment. BREC treated with IGF-I grew more rapidly than vehicle control-treated cells (p < .01); however, recombinant bovine rbIGFBP2 inhibited IGF-I-induced proliferation. IGF-II and/or rbIGFBP2 did not affect BREC proliferation. Taken together, SCFA treatment decreased IGFBP2 and IGFBP6 expression in rumen epithelial cells, and lower expression of these IGFBP might promote rumen epithelial cell proliferation by facilitating IGF-I.
Substances chimiques
Fatty Acids, Volatile
0
Insulin-Like Growth Factor Binding Protein 2
0
Insulin-Like Growth Factor Binding Protein 3
0
Insulin-Like Growth Factor Binding Protein 6
0
RNA, Messenger
0
Insulin-Like Growth Factor I
67763-96-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13422Subventions
Organisme : Japan Society for the Promotion of Science
ID : 18H02325
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 19J12823
Pays : International
Organisme : Rural Development Administration
Pays : International
Informations de copyright
© 2020 Japanese Society of Animal Science.
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