Application of the Chromosome Image Analyzing System (CHIAS) for Straightening Cation-treated Bent Chromosomes.
calcium ion (Ca2+)
chromosome image analyzing system (CHIAS)
chromosome structure
ethylenediaminetetraacetic acid (EDTA)
magnesium ion (Mg2+)
Journal
Microscopy research and technique
ISSN: 1097-0029
Titre abrégé: Microsc Res Tech
Pays: United States
ID NLM: 9203012
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
20
03
2020
revised:
18
05
2020
accepted:
02
06
2020
pubmed:
11
7
2020
medline:
19
8
2021
entrez:
11
7
2020
Statut:
ppublish
Résumé
Divalent cations, mainly calcium and magnesium ions, are known to play a major role in the maintenance of chromosomes. The depletion of both ions using ethylenediaminetetraacetic acid (EDTA) results in a bent chromosome structure with extended arms and dispersed chromatin fibers. The importance of divalent cations for the maintenance of chromosome structure has been reported previously; nevertheless, previous studies were limited to qualitative data only. Straightening the bent image of the chromosome would provide quantitative data. Thus, this study aimed to evaluate the effects of cation depletion by the application of the Chromosome Image Analyzing System (CHIAS) to straighten bent chromosomes. Human HeLa chromosomes were treated with EDTA as a known chelating agent in order to investigate the importance of divalent cations on the maintenance of chromosome structure. Chromosomes were stained and directly observed with a fluorescence microscope. Images were then analyzed using CHIAS. The results revealed that EDTA-treated chromosomes showed longer arms than those without EDTA treatment, and most of them tended to bend-out. By straightening the image using CHIAS, the bent chromosomes were successfully straightened. The average lengths of the chromosomes treated with and without EDTA were 4.97 and 0.96 μm, respectively. These results signify the advantages of CHIAS for chromosome analysis and highlight the fundamental effects of cations on chromosome condensation.
Substances chimiques
Cations
0
Magnesium
I38ZP9992A
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1411-1416Subventions
Organisme : The International Research Collaboration Grant, Universitas Indonesia, 2019 No. NKB-1945/UN2.R3.1/HKP.05.00/2019; The Japan Science and Technology Agency (JST) (SICORP) Grant Award Number: JPMJSC17E2
Informations de copyright
© 2020 Wiley Periodicals LLC.
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