Importance of tear volume for positivity of tear matrix metalloproteinase-9 immunoassay.
Adolescent
Adult
Aged
Aged, 80 and over
Cornea
/ metabolism
Dry Eye Syndromes
/ diagnosis
Eye
/ metabolism
Female
Humans
Immunoassay
/ methods
Male
Matrix Metalloproteinase 9
/ metabolism
Middle Aged
Sjogren's Syndrome
/ diagnosis
Tears
/ metabolism
Tomography, Optical Coherence
/ methods
Young Adult
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
12
2019
accepted:
15
06
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
10
9
2020
Statut:
epublish
Résumé
The tear matrix metalloproteinase-9 (MMP-9) immunoassay (Inflammadry) exhibits variable results in dry eye (DE) patients. We investigated if the tear volume in DE patients affects the results of MMP-9 immunoassay in clinical and in vitro settings. This cross-sectional study enrolled 188 eyes of 188 DE patients. The clinical symptoms and signs of DE were assessed using the Ocular Surface Disease Index and visual analog scale, strip meniscometry, tear break-up time, and tear meniscus height (TMH), area (TMA), and depth (TMD) using swept-source optical coherence tomography and corneal and conjunctival staining scores. For quantitative evaluation, the bands produced by the InflammaDry test were analyzed with ImageJ. DE subjects were grouped according to MMP-9 positivity and TMH. The InflammaDry-positive group showed greater TMH, TMA, and TMD than the MMP-9-negative group (p < 0.05). InflammaDry test band density in the high TMH group was significantly greater than that in the low and normal TMH groups (p < 0.05). InflammaDry test band density correlated positively with TMH, TMA, and TMD (all p < 0.05). InflammaDry test results were influenced by tear volume. Low tear volume in aqueous tear-deficient DE may induce false-negative results, and reflex tearing during the test may induce false-positive results.
Identifiants
pubmed: 32649686
doi: 10.1371/journal.pone.0235408
pii: PONE-D-19-33332
pmc: PMC7351366
doi:
Substances chimiques
Matrix Metalloproteinase 9
EC 3.4.24.35
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0235408Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Ocul Surf. 2016 Apr;14(2):216-23
pubmed: 26807724
Annu Rev Cell Dev Biol. 2001;17:463-516
pubmed: 11687497
Ocul Surf. 2018 Jul;16(3):368-376
pubmed: 29772277
Int J Mol Sci. 2019 Aug 01;20(15):
pubmed: 31374809
Am J Ophthalmol. 2011 May;151(5):792-798.e1
pubmed: 21310379
Ocul Surf. 2016 Apr;14(2):189-95
pubmed: 26850527
Cornea. 2013 Feb;32(2):211-6
pubmed: 22673852
Psychol Med. 1988 Nov;18(4):1007-19
pubmed: 3078045
Optom Vis Sci. 2012 Mar;89(3):336-42
pubmed: 22282222
Ophthalmology. 2015 Jan;122(1):62-71
pubmed: 25240629
Ophthalmology. 2016 Nov;123(11):2300-2308
pubmed: 27665213
Invest Ophthalmol Vis Sci. 2013 Dec 23;54(13):8285-91
pubmed: 24255042
J Neurosci. 2001 Oct 1;21(19):7724-32
pubmed: 11567062
Cornea. 2020 Feb;39(2):234-236
pubmed: 31369458
Invest Ophthalmol Vis Sci. 2009 Jul;50(7):3203-9
pubmed: 19255163
Invest Ophthalmol Vis Sci. 2015 Feb 03;56(2):738-50
pubmed: 25648341
Transl Vis Sci Technol. 2018 Oct 29;7(5):31
pubmed: 30386683
Cornea. 2000 Jul;19(4):483-6
pubmed: 10928763
JAMA Ophthalmol. 2013 Jan;131(1):24-8
pubmed: 23307206
Klin Monbl Augenheilkd. 2018 May;235(5):597-602
pubmed: 28147407
Br J Ophthalmol. 2016 Mar;100(3):300-6
pubmed: 26781133
Essays Biochem. 2016 Jun 30;60(1):111-20
pubmed: 27365041
Cornea. 2014 Aug;33(8):812-8
pubmed: 24977985
Curr Protoc Mol Biol. 2013;Chapter 14:Unit14.15
pubmed: 23547012
Arch Ophthalmol. 2000 May;118(5):615-21
pubmed: 10815152
Cornea. 2003 Oct;22(7):640-50
pubmed: 14508260