Chaetocin Improves Pig Cloning Efficiency by Enhancing Epigenetic Reprogramming and Autophagic Activity.
5-Methylcytosine
/ metabolism
Animals
Animals, Genetically Modified
/ genetics
Autophagy
/ drug effects
Blastocyst
/ physiology
Cellular Reprogramming
/ drug effects
Cloning, Organism
/ methods
DNA (Cytosine-5-)-Methyltransferases
/ genetics
DNA Methylation
/ genetics
Embryo, Mammalian
/ physiology
Embryonic Development
/ genetics
Epigenesis, Genetic
/ drug effects
Epigenomics
/ methods
Histone Deacetylase Inhibitors
/ pharmacology
Histones
/ genetics
Nuclear Transfer Techniques
Piperazines
/ pharmacology
RNA, Messenger
/ genetics
Swine
DNA methylation
H3K9me3
autophagy
chaetocin
epigenetic reprogramming
porcine SCNT embryo
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
08 Jul 2020
08 Jul 2020
Historique:
received:
11
06
2020
revised:
04
07
2020
accepted:
07
07
2020
entrez:
12
7
2020
pubmed:
12
7
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Efficient epigenetic reprogramming is crucial for the in vitro development of mammalian somatic cell nuclear transfer (SCNT) embryos. The aberrant levels of histone H3 lysine 9 trimethylation (H3K9me3) is an epigenetic barrier. In this study, we evaluated the effects of chaetocin, an H3K9me3-specific methyltransferase inhibitor, on the epigenetic reprogramming and developmental competence of porcine SCNT embryos. The SCNT embryos showed abnormal levels of H3K9me3 at the pronuclear, two-cell, and four-cell stages compared to in vitro fertilized embryos. Moreover, the expression levels of H3K9me3-specific methyltransferases (
Identifiants
pubmed: 32650566
pii: ijms21144836
doi: 10.3390/ijms21144836
pmc: PMC7402317
pii:
doi:
Substances chimiques
Histone Deacetylase Inhibitors
0
Histones
0
Piperazines
0
RNA, Messenger
0
chaetocin
28097-03-2
5-Methylcytosine
6R795CQT4H
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Korea Research Institute of Bioscience and Biotechnology
ID : KGM4252021
Organisme : National Research Foundation of Korea
ID : 2018M3A9H1023142
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