Assessment of SARS-CoV-2 in human semen-a cohort study.
ART
COVID-19
SARS-CoV-2
infertility
semen
Journal
Fertility and sterility
ISSN: 1556-5653
Titre abrégé: Fertil Steril
Pays: United States
ID NLM: 0372772
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
07
05
2020
revised:
22
05
2020
accepted:
25
05
2020
pubmed:
12
7
2020
medline:
12
8
2020
entrez:
12
7
2020
Statut:
ppublish
Résumé
To investigate the presence of viral RNA in human semen of patients with severe acute-respiratory syndrome coronavirus 2 (SARS-CoV-2) and to evaluate its presence and relevance in semen parameters. Pilot cohort study. University hospital. Thirty-four men were distributed as: 1) patients in convalescence (patients with confirmed SARS-CoV-2 infection in pharyngeal swab according to reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); 2) negative control group (no antibodies); and 3) patients with an acute infection (detection of SARS-CoV-2 in pharyngeal swab). Semen and a blood sample were collected from each individual. Analysis of semen quality according to the World Health Organization standards. Detection of SARS-CoV-2 by RT-PCR in the native semen sample and after density gradient preparation. Confirmation of immunoglobulin (Ig) A und IgG antibodies in the blood. Eighteen semen samples from recovered men were obtained 8-54 days after absence of symptoms, 14 from control subjects, and 2 from patients with an active COVID-19 infection. No RNA was detected by means of RT-PCR in the semen, including semen samples from two patients with an acute COVID-19 infection. Subjects with a moderate infection showed an impairment of sperm quality. A mild COVID-19 infection is not likely to affect testis and epididymis function, whereas semen parameters did seem impaired after a moderate infection. SARS-CoV-2 RNA could not be detected in semen of recovered and acute COVID-19-positive men. This suggests no viral transmission during sexual contact and assisted reproductive techniques, although further data need to be obtained.
Identifiants
pubmed: 32650948
pii: S0015-0282(20)30519-7
doi: 10.1016/j.fertnstert.2020.05.028
pmc: PMC7256599
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
233-238Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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