Direct Comparison of SARS-CoV-2 Analytical Limits of Detection across Seven Molecular Assays.
SARS-CoV-2
limit of detection
Journal
Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564
Informations de publication
Date de publication:
24 08 2020
24 08 2020
Historique:
received:
17
06
2020
accepted:
09
07
2020
pubmed:
12
7
2020
medline:
9
9
2020
entrez:
12
7
2020
Statut:
epublish
Résumé
Analytical sensitivity for SARS-CoV-2 detection is a key performance metric for the evaluation of viral detection assays. We determined analytical limits of detection for seven SARS-CoV-2 assays using serial dilutions of pooled patient material quantified with droplet digital PCR. Limits of detection ranged from ≤10 to 74 copies/ml for commercial high-throughput laboratory analyzers (Roche Cobas, Abbott m2000, and Hologic Panther Fusion) and 167 to 511 copies/ml for sample-to-answer (DiaSorin Simplexa, GenMark ePlex) and point-of-care instruments (Abbott ID NOW). The CDC assay yielded limits of detection ranging from 85 to 499 copies/ml, depending on the extraction method and thermocycler used. These results can help to inform the assay choice for testing approaches to manage the current COVID-19 outbreak.
Identifiants
pubmed: 32651238
pii: JCM.01535-20
doi: 10.1128/JCM.01535-20
pmc: PMC7448668
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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