Effect of Ag-1031 on apoptosis in gastric cancer AGS cells and its effects on the PI3K/AKT/mTOR signaling pathway.
Antineoplastic Agents
/ pharmacology
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Gene Expression Regulation, Neoplastic
Humans
Organic Chemicals
/ pharmacology
Phosphatidylinositol 3-Kinases
/ metabolism
Phosphorylation
/ drug effects
Proto-Oncogene Proteins c-akt
/ metabolism
Signal Transduction
/ drug effects
Stomach Neoplasms
/ drug therapy
TOR Serine-Threonine Kinases
/ metabolism
Ag-1031
Apoptosis
Gastric cancer
PI3K/AKT/mTOR signaling pathway
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
01
04
2020
accepted:
28
06
2020
pubmed:
12
7
2020
medline:
6
7
2021
entrez:
12
7
2020
Statut:
ppublish
Résumé
To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway. Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method. Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR. In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.
Identifiants
pubmed: 32651704
doi: 10.1007/s10529-020-02954-6
pii: 10.1007/s10529-020-02954-6
doi:
Substances chimiques
AG-1031
0
Antineoplastic Agents
0
Organic Chemicals
0
MTOR protein, human
EC 2.7.1.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
TOR Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2447-2452Références
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