Low protein intake during reproduction compromises the recovery of lactation-induced bone loss in female mouse dams without affecting skeletal muscles.
Animal Nutritional Physiological Phenomena
/ physiology
Animals
Animals, Newborn
Body Weight
Bone Diseases, Metabolic
/ genetics
Diet, Protein-Restricted
Female
Gene Expression Profiling
Humans
Lactation
/ physiology
Mice, Transgenic
MicroRNAs
/ genetics
Muscle, Skeletal
/ metabolism
Osteoblasts
/ metabolism
Osteogenesis
/ genetics
Reproduction
/ physiology
Weaning
bone loss
lactation
microRNAs
protein restriction
recovery
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
06
05
2020
revised:
10
06
2020
accepted:
22
06
2020
pubmed:
12
7
2020
medline:
23
3
2021
entrez:
12
7
2020
Statut:
ppublish
Résumé
Lactation-induced bone loss occurs due to high calcium requirements for fetal growth but skeletal recovery is normally achieved promptly postweaning. Dietary protein is vital for fetus and mother but the effects of protein undernutrition on the maternal skeleton and skeletal muscles are largely unknown. We used mouse dams fed with normal (N, 20%) or low (L, 8%) protein diet during gestation and lactation and maintained on the same diets (NN, LL) or switched from low to normal (LN) during a 28 d skeletal restoration period post lactation. Skeletal muscle morphology and neuromuscular junction integrity was not different between any of the groups. However, dams fed the low protein diet showed extensive bone loss by the end of lactation, followed by full skeletal recovery in NN dams, partial recovery in LN and poor bone recovery in LL dams. Primary osteoblasts from low protein diet fed mice showed decreased in vitro bone formation and decreased osteogenic marker gene expression; promoter methylation analysis by pyrosequencing showed no differences in Bmpr1a, Ptch1, Sirt1, Osx, and Igf1r osteoregulators, while miR-26a, -34a, and -125b expression was found altered in low protein fed mice. Therefore, normal protein diet is indispensable for maternal musculoskeletal health during the reproductive period.
Identifiants
pubmed: 32652768
doi: 10.1096/fj.202001131R
doi:
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11844-11859Subventions
Organisme : Medical Research Council
ID : MR/P020941/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P008429/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00014/4
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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