Nifurtimox response of
Caracas
Chagas disease
Trypanosoma cruzi
nifurtimox
oral transmission
resistance
susceptibility assay
Journal
Journal of vector borne diseases
ISSN: 0972-9062
Titre abrégé: J Vector Borne Dis
Pays: India
ID NLM: 101212761
Informations de publication
Date de publication:
Historique:
entrez:
14
7
2020
pubmed:
14
7
2020
medline:
13
8
2020
Statut:
ppublish
Résumé
In Venezuela, Chagas disease (ChD) is considered a serious health problem, with about 6 million people at risk; and acute outbreaks due to oral transmission of Chagas Disease (OChD) are becoming increasingly important. In 2007 there was a major outbreak of OChD and although patients from this episode were treated with nifurtimox (Lampit®-Bayer), about 70% therapeutic failure was registered. These results led us to examine whether parasite's drug susceptibility was related to this therapeutic failure. The Trypanosoma cruzi parasites were isolated by haemoculture of the peripheral blood drawn from the pre- and post-nifurtimox treated patients infected in the 2007 OChD outbreak at Caracas, Venezuela. The in vitro assays for drug testing were performed by the MTT methodology followed by calculation of inhibitory concentration-50 (IC Parasite isolates obtained from the infected patients prior and after nifurtimox treatment when subjected to variable concentrations of the drug showed great heterogeneity in susceptibility with IC The high heterogeneity in nifurtimox IC
Sections du résumé
BACKGROUND & OBJECTIVES
OBJECTIVE
In Venezuela, Chagas disease (ChD) is considered a serious health problem, with about 6 million people at risk; and acute outbreaks due to oral transmission of Chagas Disease (OChD) are becoming increasingly important. In 2007 there was a major outbreak of OChD and although patients from this episode were treated with nifurtimox (Lampit®-Bayer), about 70% therapeutic failure was registered. These results led us to examine whether parasite's drug susceptibility was related to this therapeutic failure.
METHODS
METHODS
The Trypanosoma cruzi parasites were isolated by haemoculture of the peripheral blood drawn from the pre- and post-nifurtimox treated patients infected in the 2007 OChD outbreak at Caracas, Venezuela. The in vitro assays for drug testing were performed by the MTT methodology followed by calculation of inhibitory concentration-50 (IC
RESULTS
RESULTS
Parasite isolates obtained from the infected patients prior and after nifurtimox treatment when subjected to variable concentrations of the drug showed great heterogeneity in susceptibility with IC
INTERPRETATION & CONCLUSION
CONCLUSIONS
The high heterogeneity in nifurtimox IC
Identifiants
pubmed: 32655073
pii: JVectorBorneDis_2019_56_3_237_289397
doi: 10.4103/0972-9062.289397
doi:
Substances chimiques
Trypanocidal Agents
0
Nifurtimox
M84I3K7C2O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
237-243Déclaration de conflit d'intérêts
None