Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage.
ASC
NMJ
glycerol
regeneration
skeletal muscle
Journal
Frontiers in molecular neuroscience
ISSN: 1662-5099
Titre abrégé: Front Mol Neurosci
Pays: Switzerland
ID NLM: 101477914
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
01
2020
accepted:
20
05
2020
entrez:
14
7
2020
pubmed:
14
7
2020
medline:
14
7
2020
Statut:
epublish
Résumé
Amongst other approaches, adipose-derived stromal cells (ASCs) have recently been tested with respect to their regenerative capacity for treatment of neuromuscular disorders. While beneficial effects of ASCs on muscle recovery were observed previously, their impact on regeneration of neuromuscular junctions (NMJs) is unclear. Here, we used a murine glycerol damage model to study disruption and regeneration of NMJs and to evaluate the effects of systemic application of ASCs on muscle and NMJ recovery. In mice that were not treated with ASCs, a differential response of NMJ pre- and post-synapses to glycerol-induced damage was observed. While post-synapses were still present in regions that were necrotic and lacking actin and dystrophin, pre-synapses disappeared soon in those affected areas. Partial regeneration of NMJs occurred within 11 days after damage. ASC treatment slightly enhanced NMJ recovery and reduced the loss of presynaptic sites, but also led to a late phase of muscle necrosis and fibrosis. In summary, the results suggest a differential sensitivity of NMJ pre- and post-synapses to glycerol-induced muscle damage and that the use of ASC for the treatment of neuromuscular disorders needs further careful evaluation.
Identifiants
pubmed: 32655366
doi: 10.3389/fnmol.2020.00107
pmc: PMC7324987
doi:
Types de publication
Journal Article
Langues
eng
Pagination
107Informations de copyright
Copyright © 2020 Rigon, Hörner, Straka, Bieback, Gretz, Hafner and Rudolf.
Références
Cell Death Dis. 2015 Apr 23;6:e1730
pubmed: 25906153
Muscle Nerve. 2013 Mar;47(3):372-8
pubmed: 23382102
Dis Model Mech. 2019 Oct 24;13(2):
pubmed: 31658990
Blood. 2001 Mar 15;97(6):1703-11
pubmed: 11238111
Stem Cells. 2013 Feb;31(2):384-96
pubmed: 23169615
Acta Neuropathol. 1990;80(2):192-7
pubmed: 2389683
J Exp Biol. 1980 Dec;89:31-42
pubmed: 7009777
Regen Med. 2017 Mar;12(2):153-167
pubmed: 28244825
Regen Med. 2014;9(4):437-52
pubmed: 25159062
Arterioscler Thromb Vasc Biol. 2020 Jan;40(1):239-254
pubmed: 31665908
Sci Rep. 2019 May 8;9(1):7090
pubmed: 31068635
J Exp Med. 2007 May 14;204(5):1057-69
pubmed: 17485518
Cell Tissue Res. 2014 May;356(2):427-43
pubmed: 24723230
Stem Cells. 2006 May;24(5):1294-301
pubmed: 16410387
Muscle Nerve. 2011 Sep;44(3):318-31
pubmed: 21949456
Acta Neuropathol. 2000 Dec;100(6):618-26
pubmed: 11078213
J Physiol. 2007 Jul 15;582(Pt 2):825-41
pubmed: 17478534
Cell Transplant. 2012;21(7):1407-17
pubmed: 23168016
Front Physiol. 2019 Nov 29;10:1416
pubmed: 31849692
Toxicon. 1996 Feb;34(2):201-11
pubmed: 8711754
Matrix Biol. 2017 Jan;57-58:86-105
pubmed: 27614294
Ann Anat. 2015 Nov;202:18-27
pubmed: 26340019
Biochem Biophys Res Commun. 2010 Jun 4;396(3):767-73
pubmed: 20457129
Stem Cell Res Ther. 2019 Apr 5;10(1):116
pubmed: 30953537
Skelet Muscle. 2016 Oct 5;6:31
pubmed: 27713817
Biol Rev Camb Philos Soc. 2017 Aug;92(3):1389-1405
pubmed: 27296513
J Endocrinol. 2016 Jun;229(3):267-75
pubmed: 27048232
Biomed Res Int. 2015;2015:453748
pubmed: 25834818
J Exp Med. 2005 May 2;201(9):1397-405
pubmed: 15867092
Exp Cell Res. 1986 Mar;163(1):211-20
pubmed: 3002828
Neurosci Res. 2004 Mar;48(3):305-14
pubmed: 15154676
Am J Physiol Heart Circ Physiol. 2008 Apr;294(4):H1550-61
pubmed: 18263710
Sci Rep. 2016 Jun 10;6:27804
pubmed: 27283349
Int J Mol Sci. 2019 Jun 24;20(12):
pubmed: 31238604
Prog Brain Res. 1979;49:325-34
pubmed: 390609
J Neurosci. 2018 Apr 25;38(17):4243-4258
pubmed: 29626165
J Cell Biol. 2007 Apr 9;177(1):73-86
pubmed: 17420291
J Cell Sci. 1999 Nov;112 ( Pt 22):4031-9
pubmed: 10547363
Front Aging Neurosci. 2015 Jul 13;7:123
pubmed: 26217219
Stem Cell Res Ther. 2018 Jul 17;9(1):195
pubmed: 30016973
Curr Med Sci. 2019 Jun;39(3):419-425
pubmed: 31209813
Micron. 2016 Dec;91:29-40
pubmed: 27710777
Sci Rep. 2016 Dec 07;6:38371
pubmed: 27924830
Folia Histochem Cytobiol. 2012 Apr 24;50(1):144-53
pubmed: 22532150
Development. 2011 Sep;138(17):3625-37
pubmed: 21828091
Physiol Rep. 2015 Apr;3(4):
pubmed: 25921779
Mol Ther. 2004 Nov;10(5):844-54
pubmed: 15509502
J Pharmacol Exp Ther. 2012 Apr;341(1):126-36
pubmed: 22228809
Exp Cell Res. 2010 Nov 1;316(18):3050-8
pubmed: 20570674
Int J Mol Sci. 2019 Jun 25;20(12):
pubmed: 31242644
PLoS One. 2013;8(3):e59622
pubmed: 23544078
Front Physiol. 2017 Apr 06;8:207
pubmed: 28428759
Front Pharmacol. 2018 Aug 08;9:917
pubmed: 30135658
Cytotherapy. 2012 May;14(5):570-83
pubmed: 22300364
Sci Rep. 2016 May 31;6:24708
pubmed: 27241590
Cell Tissue Res. 2018 Nov;374(2):233-241
pubmed: 29754258
Int J Mol Sci. 2019 Oct 31;20(21):
pubmed: 31683627
PLoS One. 2016 Jan 25;11(1):e0147198
pubmed: 26807982
Development. 2011 Sep;138(17):3657-66
pubmed: 21828094
Cell Stem Cell. 2016 Feb 4;18(2):243-52
pubmed: 26686466