Utility of smear microscopy and GeneXpert for the detection of
GeneXpert
Mycobacterium tuberculosis
Tuberculosis
culture
molecular diagnostic method
non-tuberculous mycobacteria
staining
Journal
Germs
ISSN: 2248-2997
Titre abrégé: Germs
Pays: Romania
ID NLM: 101596099
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
24
10
2019
revised:
23
01
2020
revised:
04
03
2020
accepted:
03
04
2020
entrez:
14
7
2020
pubmed:
14
7
2020
medline:
14
7
2020
Statut:
epublish
Résumé
Rapid identification of In this descriptive cross-sectional study, we compared the performance of GeneXpert in pulmonary (N=127) and extrapulmonary (N=48) clinical specimens with other diagnostic methods like culture, Auramine O (AO), and Ziehl Neelsen (ZN) staining. Rifampicin resistance was detected only by GeneXpert. Demographic data and clinical history of the subjects were collected from the patient's hospital records. AO and ZN staining when compared with mycobacterial growth indicator (MGIT) culture showed the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of 68.6, 95.7, 80, 92.4, 90.3% and 65.7, 95.7, 79.3, 91.8, 89.7%, respectively. The sensitivity, specificity, PPV, NPV and accuracy of GeneXpert was 88.6, 93.6, 77.5, 97.0 and 92.6%, respectively. GeneXpert is the best available rapid diagnostic method as it can detect MTB and rifampicin resistance gene simultaneously. Accuracy and negative predictive value of GeneXpert was found to be better than AFB staining. Thus, a negative GeneXpert test can rule out TB. Further, a negative GeneXpert and a positive smear microscopy results indicate the presence of NTM. However, GeneXpert is expensive and needs sophisticated instrument when compared to smear microscopy.
Identifiants
pubmed: 32656104
doi: 10.18683/germs.2020.1188
pii: germs.2020.1188
pmc: PMC7330512
doi:
Types de publication
Journal Article
Langues
eng
Pagination
81-87Informations de copyright
GERMS.
Déclaration de conflit d'intérêts
Conflicts of interest: All authors – none to declare.
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