Whole blood viscosity and red blood cell adhesion: Potential biomarkers for targeted and curative therapies in sickle cell disease.
Journal
American journal of hematology
ISSN: 1096-8652
Titre abrégé: Am J Hematol
Pays: United States
ID NLM: 7610369
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
01
07
2020
revised:
07
07
2020
accepted:
08
07
2020
pubmed:
14
7
2020
medline:
30
12
2020
entrez:
14
7
2020
Statut:
ppublish
Résumé
Sickle cell disease (SCD) is a recessive genetic blood disorder exhibiting abnormal blood rheology. Polymerization of sickle hemoglobin, due to a point mutation in the β-globin gene of hemoglobin, results in aberrantly adhesive and stiff red blood cells (RBCs). Hemolysis, abnormal RBC adhesion, and abnormal blood rheology together impair endothelial health in people with SCD, which leads to cumulative systemic complications. Here, we describe a microfluidic assay combined with a micro particle image velocimetry technique for the integrated in vitro assessment of whole blood viscosity (WBV) and RBC adhesion. We examined WBV and RBC adhesion to laminin (LN) in microscale flow in whole blood samples from 53 individuals with no hemoglobinopathies (HbAA, N = 10), hemoglobin SC disease (HbSC, N = 14), or homozygous SCD (HbSS, N = 29) with mean WBV of 4.50 cP, 4.08 cP, and 3.73 cP, respectively. We found that WBV correlated with RBC count and hematocrit in subjects with HbSC or HbSS. There was a significant inverse association between WBV and RBC adhesion under both normoxic and physiologically hypoxic (SpO
Identifiants
pubmed: 32656816
doi: 10.1002/ajh.25933
pmc: PMC7689825
mid: NIHMS1625777
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1246-1256Subventions
Organisme : NHLBI NIH HHS
ID : U01 HL117659
Pays : United States
Organisme : NHLBI NIH HHS
ID : OT2HL152643
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL134622
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133574
Pays : United States
Organisme : NHLBI NIH HHS
ID : OT2 HL152643
Pays : United States
Informations de copyright
© 2020 The Authors. American Journal of Hematology published by Wiley Periodicals LLC.
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