Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism.
Drosophila
cell polarity
endocytosis
membrane traffic
sorting
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
17 08 2020
17 08 2020
Historique:
received:
18
04
2020
revised:
10
06
2020
accepted:
16
06
2020
pubmed:
14
7
2020
medline:
7
4
2021
entrez:
14
7
2020
Statut:
ppublish
Résumé
Plasma membranes fulfil many physiological functions. In polarized cells, different membrane compartments take on specialized roles, each being allocated correct amounts of membrane. The Drosophila tracheal system, an established tubulogenesis model, contains branched terminal cells with subcellular tubes formed by apical plasma membrane invagination. We show that apical endocytosis and late endosome-mediated trafficking are required for membrane allocation to the apical and basal membrane domains. Basal plasma membrane growth stops if endocytosis is blocked, whereas the apical membrane grows excessively. Plasma membrane is initially delivered apically and then continuously endocytosed, together with apical and basal cargo. We describe an organelle carrying markers of late endosomes and multivesicular bodies (MVBs) that is abolished by inhibiting endocytosis and which we suggest acts as transit station for membrane destined to be redistributed both apically and basally. This is based on the observation that disrupting MVB formation prevents growth of both compartments.
Identifiants
pubmed: 32657472
doi: 10.15252/embj.2020105332
pmc: PMC7429744
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e105332Subventions
Organisme : European Molecular Biology Laboratory (EMBL)
Organisme : European Molecular Biology Organization (EMBO)
Organisme : EMBL Interdisciplinary Postdoctoral Programme under Marie Curie Actions
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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