Viral metagenomics analysis of kidney donors and recipients: Torque teno virus genotyping and prevalence.
Anelloviridae
immunocompromised patients
next-generation sequencing
solid organ transplantation
torque teno virus
viral metagenomics
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
25
02
2020
accepted:
07
07
2020
medline:
14
7
2020
pubmed:
14
7
2020
entrez:
14
7
2020
Statut:
ppublish
Résumé
The viral load of the ubiquitous and nonpathogenic torque teno virus (TTV) is associated with the grade of immunosuppression of its host. The development of next-generation sequencing (NGS) allowed to describe the human virome of the blood compartment in transplanted patients, and showed that TTV is the most important part of the virome. This study is a descriptive retrospective pilot study of sequencing plasma samples from 15 matched donors and recipients. After sample processing, nucleic acids were amplified by rolling circle amplification and submitted to NGS by ion proton sequencing technology. Results were analyzed after mapping of reads on the 29 TTV reference genomes and de novo assembling of the reads with MIRA software. The number of TTV species present in donors and recipients was, on average, 12 in donors and 33 in recipients. TTV species predominantly present in donors were TTV-13 and TTV-18; and in recipients were TTV-P15-2, TTV-27, TTV-HD14a, and TTV-22. We highlighted a significant variability in abundance and composition in sequential samples from recipients. Temporal evolution of TTV populations was clearly observed in recipients, but no preferential transmission of a species from donor to recipient was evidenced. Diversity and population expansion were observed in kidney recipients. Further study of TTV species could help assess the potential impact of each species of this virus.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3301-3311Subventions
Organisme : Limoges Teacher Hospital clinical Research Department
ID : APREL Grant 2015
Organisme : bioMérieux SA
Organisme : Institut National de la Santé et de la Recherche Médicale
Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
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