Immunoglobulin fragment F(ab')
Animals
Antibodies, Neutralizing
/ immunology
Antibodies, Viral
/ immunology
Betacoronavirus
/ immunology
COVID-19
Chlorocebus aethiops
Coronavirus Infections
/ therapy
Female
HeLa Cells
Humans
Mice, Inbred BALB C
Neutralization Tests
Pandemics
Pneumonia, Viral
/ therapy
Protein Binding
Receptors, Immunologic
/ immunology
SARS-CoV-2
Spike Glycoprotein, Coronavirus
/ immunology
Vero Cells
COVID-19
Immunoglobulin fragment
Neutralizing antibody
Receptor-binding domain
SARS-CoV-2
Journal
Antiviral research
ISSN: 1872-9096
Titre abrégé: Antiviral Res
Pays: Netherlands
ID NLM: 8109699
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
15
05
2020
revised:
28
06
2020
accepted:
30
06
2020
pubmed:
14
7
2020
medline:
27
10
2020
entrez:
14
7
2020
Statut:
ppublish
Résumé
COVID-19, which is caused by the emerging human coronavirus SARS-CoV-2, has become a global pandemic that poses a serious threat to human health. To date, no vaccines or specific antiviral drugs have been approved for the treatment of this disease in clinic. Herein, therapeutic antibodies for SARS-CoV-2 were obtained from hyperimmune equine plasma. First, a recombinant SARS-CoV-2 spike protein receptor-binding domain (RBD) was obtained in gram-level quantities through high-cell density fermentation of Chinese hamster ovary cells. Then, the binding of the RBD to the SARS-CoV-2 receptor, human angiotensin-converting enzyme 2, was verified by several biochemical methods. The efficacy of the RBD in triggering antibody response in vivo was subsequently tested in both mice and equines, and the results showed that the RBD triggered high-titer neutralizing antibody production in vivo. Immunoglobulin F(ab')
Identifiants
pubmed: 32659292
pii: S0166-3542(20)30282-5
doi: 10.1016/j.antiviral.2020.104868
pmc: PMC7351055
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
F(ab')2 receptor
0
Receptors, Immunologic
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104868Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
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