Diminished expression of 5hmc in Reed-Sternberg cells in classical Hodgkin lymphoma is a common epigenetic marker.


Journal

Leukemia research
ISSN: 1873-5835
Titre abrégé: Leuk Res
Pays: England
ID NLM: 7706787

Informations de publication

Date de publication:
09 2020
Historique:
received: 13 02 2019
revised: 28 04 2020
accepted: 28 06 2020
pubmed: 14 7 2020
medline: 15 12 2020
entrez: 14 7 2020
Statut: ppublish

Résumé

Loss of the epigenetic marker 5-hydroxymethylcytosine (5hmC) has been demonstrated in a variety of neoplasms. Several recent studies have shown epigenetic alteration in Classical Hodgkin lymphoma (CHL), which may impact treatment. We demonstrate near universal depletion of 5hmC in the neoplastic Hodgkin Reed-Sternberg (H/RS) cells in all cases of CHL (49/49). We hypothesized that the addition of vitamin C-a cofactor for the ten-eleven translocation (TET) enzymes which oxidize 5-methylcytosine (5mC) to 5hmC - may replenish levels of 5hmC. The CHL cell line L428 was grown in optimal conditions and then subjected to vitamin C treatment, which demonstrated reduced cell viability as well as caspase activation and increased concentration of 5hmC. A more detailed understanding of the epigenetic landscape of CHL may help guide future therapies.

Identifiants

pubmed: 32659407
pii: S0145-2126(20)30113-2
doi: 10.1016/j.leukres.2020.106408
pii:
doi:

Substances chimiques

Biomarkers 0
5-hydroxymethylcytosine 1123-95-1
5-Methylcytosine 6R795CQT4H

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106408

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Andrew Siref (A)

Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, 8700 Beverly Blvd., Room #4711, Los Angeles, CA 90048-1804, United States.

Colin McCormack (C)

Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, 8700 Beverly Blvd., Room #4711, Los Angeles, CA 90048-1804, United States.

Qin Huang (Q)

Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, 8700 Beverly Blvd., Room #4711, Los Angeles, CA 90048-1804, United States.

Washington Lim (W)

Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, 8700 Beverly Blvd., Room #4711, Los Angeles, CA 90048-1804, United States.

Serhan Alkan (S)

Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, 8700 Beverly Blvd., Room #4711, Los Angeles, CA 90048-1804, United States. Electronic address: Serhan.Alkan@cshs.org.

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Classifications MeSH