Insights into mRNA degradation from single-molecule imaging in living cells.
Journal
Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
19
03
2020
revised:
15
05
2020
accepted:
02
06
2020
pubmed:
14
7
2020
medline:
26
8
2021
entrez:
14
7
2020
Statut:
ppublish
Résumé
Single-molecule fluorescence microscopy techniques have enabled the lifecycle of individual RNA transcripts to be quantitatively measured in living cells. The application of these approaches to monitor mRNA degradation, however, has presented a challenge to unequivocally detect these events due to the inherent loss-of-signal resulting from decay of a transcript. Here, we highlight the recent technological developments that have enabled the spatial and temporal dynamics of mRNA degradation of individual transcripts to be visualized within living cells.
Identifiants
pubmed: 32659634
pii: S0959-440X(20)30102-0
doi: 10.1016/j.sbi.2020.06.003
pii:
doi:
Substances chimiques
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
89-95Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.