A Multimethod, Multicountry Evaluation of Breakpoints for Bedaquiline Resistance Determination.


Journal

Antimicrobial agents and chemotherapy
ISSN: 1098-6596
Titre abrégé: Antimicrob Agents Chemother
Pays: United States
ID NLM: 0315061

Informations de publication

Date de publication:
20 08 2020
Historique:
received: 11 03 2020
accepted: 03 07 2020
pubmed: 15 7 2020
medline: 22 6 2021
entrez: 15 7 2020
Statut: epublish

Résumé

Criteria defining bedaquiline resistance for tuberculosis have been proposed addressing an emerging concern. We evaluated bedaquiline phenotypic drug susceptibility testing (pDST) criteria using drug-resistant tuberculosis clinical isolates tested at five reference laboratories. Isolates were tested at the proposed bedaquiline MGIT960 and 7H11 agar proportion (AP) critical concentrations and also at higher dilutions. The epidemiological cutoff value for the broth microdilution (BMD) plates (frozen and dry) was investigated. Sanger sequencing was performed (

Identifiants

pubmed: 32660992
pii: AAC.00479-20
doi: 10.1128/AAC.00479-20
pmc: PMC7449194
pii:
doi:

Substances chimiques

Antitubercular Agents 0
Diarylquinolines 0
bedaquiline 78846I289Y

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 American Society for Microbiology.

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Auteurs

Nazir Ahmed Ismail (NA)

Center for Tuberculosis and WHO Supranational TB Reference Laboratory, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa naziri@nicd.ac.za.

Akio Aono (A)

Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, Kiyose, Japan.

Emanuele Borroni (E)

Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.

Daniela Maria Cirillo (DM)

Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.

Christel Desmaretz (C)

Department of Biomedical Sciences, Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.

Rumina Hasan (R)

Department of Pathology and Laboratory Medicine, The Aga Khan University, Karachi, Pakistan.
Faculty of Infectious and Tropical Disease, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Satoshi Mitarai (S)

Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, Kiyose, Japan.

Sadia Shakoor (S)

Department of Pathology and Laboratory Medicine, The Aga Khan University, Karachi, Pakistan.

Gabriela Torrea (G)

Department of Biomedical Sciences, Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.

Koné Kaniga (K)

Johnson & Johnson Global Public Health, Titusville, New Jersey, USA.

Shaheed V Omar (SV)

Center for Tuberculosis and WHO Supranational TB Reference Laboratory, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.

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Classifications MeSH