Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers.
COVID-19
SARS-CoV-2
WBE
aircraft
cruise ship
enveloped viruses
wastewater
Journal
Journal of travel medicine
ISSN: 1708-8305
Titre abrégé: J Travel Med
Pays: England
ID NLM: 9434456
Informations de publication
Date de publication:
20 08 2020
20 08 2020
Historique:
received:
15
06
2020
revised:
30
06
2020
accepted:
06
07
2020
pubmed:
15
7
2020
medline:
4
9
2020
entrez:
15
7
2020
Statut:
ppublish
Résumé
Wastewater-based epidemiology (WBE) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be an important source of information for coronavirus disease 2019 (COVID-19) management during and after the pandemic. Currently, governments and transportation industries around the world are developing strategies to minimize SARS-CoV-2 transmission associated with resuming activity. This study investigated the possible use of SARS-CoV-2 RNA wastewater surveillance from airline and cruise ship sanitation systems and its potential use as a COVID-19 public health management tool. Aircraft and cruise ship wastewater samples (n = 21) were tested for SARS-CoV-2 using two virus concentration methods, adsorption-extraction by electronegative membrane (n = 13) and ultrafiltration by Amicon (n = 8), and five assays using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and RT-droplet digital PCR (RT-ddPCR). Representative qPCR amplicons from positive samples were sequenced to confirm assay specificity. SARS-CoV-2 RNA was detected in samples from both aircraft and cruise ship wastewater; however concentrations were near the assay limit of detection. The analysis of multiple replicate samples and use of multiple RT-qPCR and/or RT-ddPCR assays increased detection sensitivity and minimized false-negative results. Representative qPCR amplicons were confirmed for the correct PCR product by sequencing. However, differences in sensitivity were observed among molecular assays and concentration methods. The study indicates that surveillance of wastewater from large transport vessels with their own sanitation systems has potential as a complementary data source to prioritize clinical testing and contact tracing among disembarking passengers. Importantly, sampling methods and molecular assays must be further optimized to maximize detection sensitivity. The potential for false negatives by both wastewater testing and clinical swab testing suggests that the two strategies could be employed together to maximize the probability of detecting SARS-CoV-2 infections amongst passengers.
Sections du résumé
BACKGROUND
Wastewater-based epidemiology (WBE) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be an important source of information for coronavirus disease 2019 (COVID-19) management during and after the pandemic. Currently, governments and transportation industries around the world are developing strategies to minimize SARS-CoV-2 transmission associated with resuming activity. This study investigated the possible use of SARS-CoV-2 RNA wastewater surveillance from airline and cruise ship sanitation systems and its potential use as a COVID-19 public health management tool.
METHODS
Aircraft and cruise ship wastewater samples (n = 21) were tested for SARS-CoV-2 using two virus concentration methods, adsorption-extraction by electronegative membrane (n = 13) and ultrafiltration by Amicon (n = 8), and five assays using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and RT-droplet digital PCR (RT-ddPCR). Representative qPCR amplicons from positive samples were sequenced to confirm assay specificity.
RESULTS
SARS-CoV-2 RNA was detected in samples from both aircraft and cruise ship wastewater; however concentrations were near the assay limit of detection. The analysis of multiple replicate samples and use of multiple RT-qPCR and/or RT-ddPCR assays increased detection sensitivity and minimized false-negative results. Representative qPCR amplicons were confirmed for the correct PCR product by sequencing. However, differences in sensitivity were observed among molecular assays and concentration methods.
CONCLUSIONS
The study indicates that surveillance of wastewater from large transport vessels with their own sanitation systems has potential as a complementary data source to prioritize clinical testing and contact tracing among disembarking passengers. Importantly, sampling methods and molecular assays must be further optimized to maximize detection sensitivity. The potential for false negatives by both wastewater testing and clinical swab testing suggests that the two strategies could be employed together to maximize the probability of detecting SARS-CoV-2 infections amongst passengers.
Identifiants
pubmed: 32662867
pii: 5871228
doi: 10.1093/jtm/taaa116
pmc: PMC7454825
pii:
doi:
Substances chimiques
RNA, Viral
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© International Society of Travel Medicine 2020. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
Références
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Appl Environ Microbiol. 2011 May;77(10):3500-6
pubmed: 21441329
Clin Chem. 2009 Apr;55(4):611-22
pubmed: 19246619
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
J Travel Med. 2017 Sep 1;24(6):
pubmed: 29088487
Syst Appl Microbiol. 2010 Oct;33(6):348-57
pubmed: 20655680
J Clin Microbiol. 2020 May 26;58(6):
pubmed: 32269100
Sci Total Environ. 2020 Oct 15;739:139076
pubmed: 32758929
Lancet Infect Dis. 2020 May;20(5):533-534
pubmed: 32087114
Water Res. 2017 Jan 1;108:25-31
pubmed: 27838026
J Clin Med. 2020 Feb 19;9(2):
pubmed: 32093043
Appl Environ Microbiol. 2012 Oct;78(20):7317-26
pubmed: 22885746
Lancet Gastroenterol Hepatol. 2020 Jun;5(6):533-534
pubmed: 32246939
Sci Total Environ. 2020 Sep 20;736:139652
pubmed: 32464333
Epidemiol Infect. 2003 Apr;130(2):263-71
pubmed: 12729195
Jpn J Infect Dis. 2020 Jul 22;73(4):304-307
pubmed: 32074516
Sci Total Environ. 2020 Oct 15;739:139960
pubmed: 32758945
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
J Travel Med. 2020 May 18;27(3):
pubmed: 32109273
Appl Environ Microbiol. 2015 Mar;81(6):2042-9
pubmed: 25576614
Appl Environ Microbiol. 2009 Mar;75(5):1402-9
pubmed: 19124584
Water Res. 2020 Aug 15;181:115942
pubmed: 32425251
Clin Microbiol Infect. 2020 Jun;26(6):773-779
pubmed: 32276116
Environ Sci Technol. 2016 Jul 5;50(13):6803-13
pubmed: 26992352
Sci Total Environ. 2020 Aug 1;728:138764
pubmed: 32387778
Lancet Infect Dis. 2020 Jun;20(6):631-633
pubmed: 32213329
BMJ. 2020 Apr 21;369:m1443
pubmed: 32317267
J Korean Med Sci. 2020 Mar 23;35(11):e123
pubmed: 32193904
Am J Gastroenterol. 2020 May;115(5):790
pubmed: 32205644
Nat Microbiol. 2020 Jul 10;:
pubmed: 32651556
Euro Surveill. 2020 Mar;25(10):
pubmed: 32183930