Over-Expression of RNA Processing, Heat Shock, and DNA Repair Proteins in Breast Tumor Compared to Normal Tissue.
breast cancer
formaldehyde-fixed paraffin-embedded
mRNA processing
mass spectrometry
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
14 Jul 2020
14 Jul 2020
Historique:
received:
19
02
2020
revised:
16
06
2020
pubmed:
15
7
2020
medline:
15
7
2020
entrez:
15
7
2020
Statut:
aheadofprint
Résumé
This study identifies the main changes in protein expression in human breast tumors compared to normal breast tissue. Malignant tumors (32) and normal breast tissue samples (23), from formaldehyde-fixed, paraffin-embedded specimens are subjected to discovery proteomics using liquid chromatography/tandem mass spectrometry, with spectral counts for quantitation. The dataset contains 1406 proteins. Differential expression is measured using a method that takes advantage of estimates of the percentage of tumor on a slide. This analysis shows that the major classes of proteins over-expressed by tumors are RNA-binding, heat shock and DNA repair proteins. RNA-binding proteins, including heterogeneous nuclear ribonucleoproteins (HNRNPs), SR splice factors (SRSF) and elongation factors form the largest group. Comparison with results from another study demonstrates that the RNA-binding proteins are associated specifically with malignant transformation, rather than with cell proliferation. HNRNP and SRSF proteins help define splice sites in normal cells. Their over-expression may dysregulate splicing, which in turn has the potential to promote malignant transformation.
Identifiants
pubmed: 32663359
doi: 10.1002/pmic.202000044
pmc: PMC7855622
mid: NIHMS1632087
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000044Subventions
Organisme : NCI NIH HHS
ID : R15 CA164929
Pays : United States
Organisme : NIH HHS
ID : R15CA164929
Pays : United States
Organisme : National Science Foundation
ID : CHE-1228656
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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