A mitochondria-targeted coenzyme Q peptoid induces superoxide dismutase and alleviates salinity stress in plant cells.
Agriculture
Food Security
Humans
Mitochondria
/ genetics
Oxidation-Reduction
Oxidative Stress
/ genetics
Peptoids
/ genetics
Plant Cells
/ enzymology
Reactive Oxygen Species
/ metabolism
Salt Stress
/ genetics
Superoxide Dismutase
/ genetics
Nicotiana
/ growth & development
Ubiquinone
/ analogs & derivatives
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 07 2020
14 07 2020
Historique:
received:
13
11
2019
accepted:
10
06
2020
entrez:
16
7
2020
pubmed:
16
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Salinity is a serious challenge to global agriculture and threatens human food security. Plant cells can respond to salt stress either by activation of adaptive responses, or by programmed cell death. The mechanisms deciding the respective response are far from understood, but seem to depend on the degree, to which mitochondria can maintain oxidative homeostasis. Using plant PeptoQ, a Trojan Peptoid, as vehicle, it is possible to transport a coenzyme Q10 (CoQ10) derivative into plant mitochondria. We show that salinity stress in tobacco BY-2 cells (Nicotiana tabacum L. cv Bright Yellow-2) can be mitigated by pretreatment with plant PeptoQ with respect to numerous aspects including proliferation, expansion, redox homeostasis, and programmed cell death. We tested the salinity response for transcripts from nine salt-stress related-genes representing different adaptive responses. While most did not show any significant response, the salt response of the transcription factor NtNAC, probably involved in mitochondrial retrograde signaling, was significantly modulated by the plant PeptoQ. Most strikingly, transcripts for the mitochondrial, Mn-dependent Superoxide Dismutase were rapidly and drastically upregulated in presence of the peptoid, and this response was disappearing in presence of salt. The same pattern, albeit at lower amplitude, was seen for the sodium exporter SOS1. The findings are discussed by a model, where plant PeptoQ modulates retrograde signalling to the nucleus leading to a strong expression of mitochondrial SOD, what renders mitochondria more resilient to perturbations of oxidative balance, such that cells escape salt induced cell death and remain viable.
Identifiants
pubmed: 32665569
doi: 10.1038/s41598-020-68491-4
pii: 10.1038/s41598-020-68491-4
pmc: PMC7360622
doi:
Substances chimiques
Peptoids
0
Reactive Oxygen Species
0
Ubiquinone
1339-63-5
Superoxide Dismutase
EC 1.15.1.1
coenzyme Q10
EJ27X76M46
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11563Références
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