IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 07 2020
14 07 2020
Historique:
received:
06
11
2019
accepted:
11
06
2020
entrez:
16
7
2020
pubmed:
16
7
2020
medline:
1
9
2020
Statut:
epublish
Résumé
It is unclear whether the establishment of apical-basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and podocalyxin. Molecularly, IRSp53 acts by regulating the localization and activity of the small GTPase RAB35, and by interacting with the actin capping protein EPS8. Using correlative light and electron microscopy, we further show that IRSp53 ensures the shape and continuity of the opposing plasma membrane of two daughter cells, leading to the formation of a single apical lumen. Genetic removal of IRSp53 results in abnormal renal tubulogenesis, with altered tubular polarity and architectural organization. Thus, IRSp53 acts as a membrane curvature-sensing platform for the assembly of multi-protein complexes that control the trafficking of apical determinants and the integrity of the luminal plasma membrane.
Identifiants
pubmed: 32665580
doi: 10.1038/s41467-020-17091-x
pii: 10.1038/s41467-020-17091-x
pmc: PMC7360740
doi:
Substances chimiques
Actins
0
BAIAP2 protein, human
0
Nerve Tissue Proteins
0
Sialoglycoproteins
0
podocalyxin
0
RAB35 protein, human
EC 3.6.1.-
rab GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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