A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2.
COVID qPCR analyzer
COVID-19 detection assay
Diagnostics
Genomics
Graphical user interface (GUI)
Infectious disease
Microbial genomics
Molecular biology
Real-time RT- PCR
SARS-CoV-2
Virology
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
15
06
2020
revised:
01
07
2020
accepted:
03
07
2020
entrez:
16
7
2020
pubmed:
16
7
2020
medline:
16
7
2020
Statut:
epublish
Résumé
Early diagnosis of SARS-CoV-2 infected patients is essential to control the dynamics of the COVID-19 pandemic. We develop a rapid and accurate one-step multiplex TaqMan probe-based real-time RT-PCR assay, along with a computational tool to systematically analyse the data. Our assay could detect to a limit of 15 copies of SARS-CoV-2 transcripts-based on experiments performed by spiking total human RNA with
Identifiants
pubmed: 32665985
doi: 10.1016/j.heliyon.2020.e04405
pii: S2405-8440(20)31249-4
pii: e04405
pmc: PMC7341355
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e04405Informations de copyright
© 2020 The Author(s).
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