Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts.
DNA methylation
Epigenetics
Human primary fibroblasts
Plant extract
Vitis vinifera L.
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
06
05
2020
accepted:
03
07
2020
pubmed:
16
7
2020
medline:
23
6
2021
entrez:
16
7
2020
Statut:
ppublish
Résumé
Adverse environmental conditions such as UV radiation induce oxidative and aging events leading to severe damage to human skin cells. Natural products such as plant extracts have been implicated in the skin anti-oxidant and anti-aging cellular protection against environmental stress. Moreover, environmental factors have been shown to impact chromatin structure leading to altered gene expression programs with profound changes in cellular functions. In this study, we assessed the in vitro effect of a leaf extract from Vitis vinifera L. on UV-stressed primary human dermal fibroblasts, focusing on gene expression and DNA methylation as an epigenetic factor. Expression analysis of two genes known to be implicated in skin anti-aging, SIRT1and HSP4, demonstrated significant induction in the presence of the extract under normal or UVA conditions. In addition, DNA methylation profiling of SIRT1 and HSP47 promoters showed that the V. vinifera L. extract induced changes in the DNA methylation pattern of both genes that may be associated with SIRT1 and HSP47 gene expression. Our study shows for the first time transcriptional and DNA methylation alterations on human skin fibroblasts exposed to UV stress and suggest a protective effect of a V. vinifera extract possibly through transcriptional regulation of critical skin anti-aging genes.
Identifiants
pubmed: 32666439
doi: 10.1007/s11033-020-05645-7
pii: 10.1007/s11033-020-05645-7
doi:
Substances chimiques
Antioxidants
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5763-5772Commentaires et corrections
Type : ErratumIn
Références
Yamaba H, Haba M, Kunita M et al (2016) Morphological change of skin fibroblasts induced by UV irradiation is involved in photoaging. Exp Dermatol 25:45–51. https://doi.org/10.1111/exd.13084
doi: 10.1111/exd.13084
pubmed: 27539902
Varani J, Schuger L, Dame MK et al (2004) Reduced fibroblast interaction with intact collagen as a mechanism for depressed collagen synthesis in photodamaged skin. J Invest Dermatol 122:1471–1479. https://doi.org/10.1111/j.0022-202X.2004.22614.x
doi: 10.1111/j.0022-202X.2004.22614.x
pubmed: 15175039
Amaro-Ortiz A, Yan B, D’Orazio J (2014) Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules 19:6202–6219. https://doi.org/10.3390/molecules19056202
doi: 10.3390/molecules19056202
pubmed: 24838074
pmcid: 4344124
Soto M, Falqué E, Domínguez H (2015) Relevance of natural phenolics from grape and derivative products in the formulation of cosmetics. Cosmetics 2:259–276. https://doi.org/10.3390/cosmetics2030259
doi: 10.3390/cosmetics2030259
Alcendor RR, Gao S, Zhai P et al (2007) Sirt1 regulates aging and resistance to oxidative stress in the heart. Circ Res 100:1512–1521. https://doi.org/10.1161/01.RES.0000267723.65696.4a
doi: 10.1161/01.RES.0000267723.65696.4a
pubmed: 17446436
Haigis MC, Guarente LP (2006) Mammalian sirtuins—emerging roles in physiology, aging, and calorie restriction. Genes Dev 20:2913–2921. https://doi.org/10.1101/gad.1467506
doi: 10.1101/gad.1467506
pubmed: 17079682
Guarente L, Picard F (2005) Calorie restriction—the SIR2 connection. Cell 120:473–482. https://doi.org/10.1016/j.cell.2005.01.029
doi: 10.1016/j.cell.2005.01.029
pubmed: 15734680
Ito S, Nagata K (2017) Biology of Hsp47 (Serpin H1), a collagen-specific molecular chaperone. Semin Cell Dev Biol 62:142–151. https://doi.org/10.1016/j.semcdb.2016.11.005
doi: 10.1016/j.semcdb.2016.11.005
pubmed: 27838364
Imai S, Armstrong CM, Kaeberlein M, Guarente L (2000) Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403:795–800. https://doi.org/10.1038/35001622
doi: 10.1038/35001622
pubmed: 10693811
Pal S, Tyler JK (2016) Epigenetics and aging. Sci Adv 2:e1600584. https://doi.org/10.1126/sciadv.1600584
doi: 10.1126/sciadv.1600584
pubmed: 27482540
pmcid: 4966880
Liang YC, Widelitz RCC (2018) Epigenetic regulation of skin development and regeneration. Springer, Cham
Pagiatakis C, Musolino E, Gornati R et al (2019) Epigenetics of aging and disease: a brief overview. Aging Clin Exp Res. https://doi.org/10.1007/s40520-019-01430-0
doi: 10.1007/s40520-019-01430-0
pubmed: 31811572
pmcid: 8084772
Lantzouraki DZ, Tsiaka T, Soteriou N et al (2020) Antioxidant profiles of Vitis vinifera L. and Salvia triloba L. leaves using high-energy extraction methodologies. J AOAC Int 103:413–421. https://doi.org/10.5740/jaoacint.19-0261
doi: 10.5740/jaoacint.19-0261
pubmed: 31530341
Hoffmann M, Wu Y-J, Gerber M et al (2010) Fusion-active glycoprotein G mediates the cytotoxicity of vesicular stomatitis virus M mutants lacking host shut-off activity. J Gen Virol 91:2782–2793. https://doi.org/10.1099/vir.0.023978-0
doi: 10.1099/vir.0.023978-0
pubmed: 20631091
Chen H, Li Y, Tollefsbol TO (2013) Cell senescence culturing methods. Methods Mol Biol. 21:1–10
Letsiou S, Kalliampakou K, Gardikis K et al (2017) Skin protective effects of nannochloropsis gaditana extract on H
Ramakers C, Ruijter JM, Deprez RHL, Moorman AF (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339:62–66. https://doi.org/10.1016/S0304-3940(02)01423-4
doi: 10.1016/S0304-3940(02)01423-4
pubmed: 12618301
Beier V, Mund C, Hoheisel JD (2006) Monitoring methylation changes in cancer. In: Analytics of protein–DNA interactions. Springer, Berlin, pp 1–11
Zhao L, Cao J, Hu K, He X, Yun D, Tong T, Han L (2020) Sirtuins and their biological relevance in aging and age-related diseases. Aging Dis 11. https://doi.org/10.14336/AD.2019.0820
Crouch SPM, Kozlowski R, Slater KJ, Fletcher J (1993) The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J Immunol Methods 160:81–88. https://doi.org/10.1016/0022-1759(93)90011-U
doi: 10.1016/0022-1759(93)90011-U
pubmed: 7680699
Deters AM, Schröder KR, Hensel A (2005) Kiwi fruit (Actinidia chinensis L.) polysaccharides exert stimulating effects on cell proliferation via enhanced growth factor receptors, energy production, and collagen synthesis of human keratinocytes, fibroblasts, and skin equivalents. J Cell Physiol 202:717–722. https://doi.org/10.1002/jcp.20161
doi: 10.1002/jcp.20161
pubmed: 15389574
Yamakuchi M (2012) MicroRNA regulation of SIRT1. Front Physiol 3. https://doi.org/10.3389/fphys.2012.00068
Elibol B, Kilic U (2018) High levels of SIRT1 expression as a protective mechanism against disease-related conditions. Front Endocrinol (Lausanne) 9. https://doi.org/10.3389/fendo.2018.00614
Kabra N, Li Z, Chen L et al (2009) SirT1 is an inhibitor of proliferation and tumor formation in colon cancer. J Biol Chem 284:18210–18217. https://doi.org/10.1074/jbc.M109.000034
doi: 10.1074/jbc.M109.000034
pubmed: 19433578
pmcid: 2709385
Satoh A, Stein L, Imai S (2011) The role of mammalian sirtuins in the regulation of metabolism, aging, and longevity. Handb Exp Pharmocol 206:125–162
doi: 10.1007/978-3-642-21631-2_7
Hubbard BP, Sinclair DA (2014) Small molecule SIRT1 activators for the treatment of aging and age-related diseases. Trends Pharmacol Sci 35:146–154. https://doi.org/10.1016/j.tips.2013.12.004
doi: 10.1016/j.tips.2013.12.004
pubmed: 24439680
pmcid: 3970218
Zhang C, Wen C, Lin J, Shen G (2015) Protective effect of pyrroloquinoline quinine on ultraviolet a irradiation-induced human dermal fibroblast senescence in vitro proceeds via the anti-apoptotic sirtuin 1/nuclear factor-derived erythroid 2-related factor 2/heme oxygenase 1 pathway. Mol Med Rep 12:4382–4388. https://doi.org/10.3892/mmr.2015.3990
doi: 10.3892/mmr.2015.3990
pubmed: 26126510
Khan ES, Sankaran S, Paez JI et al (2019) Photoactivatable hsp47: a tool to regulate collagen secretion and assembly. Adv Sci 6:1801982. https://doi.org/10.1002/advs.201801982
doi: 10.1002/advs.201801982
He W, Dai C (2015) Key fibrogenic signaling. Curr Pathobiol Rep 3(183):192. https://doi.org/10.1007/s40139-015-0077-z
doi: 10.1007/s40139-015-0077-z
Makareeva E, Leikin S (2007) Procollagen triple helix assembly: an unconventional chaperone-assisted folding paradigm. PLoS ONE 2:e1029. https://doi.org/10.1371/journal.pone.0001029
doi: 10.1371/journal.pone.0001029
pubmed: 17925877
pmcid: 2000351
Nizard C, Noblesse E, Boisdé C et al (2004) Heat shock protein 47 expression in aged normal human fibroblasts: modulation by salix alba extract. Ann N Y Acad Sci 1019:223–227. https://doi.org/10.1196/annals.1297.037
doi: 10.1196/annals.1297.037
pubmed: 15247019