Self-Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid-Liquid Phase Separation.
DNA
liquid-liquid phase separation
membraneless cell organelles
microdroplets
synthetic biology
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
29
05
2020
revised:
09
07
2020
pubmed:
16
7
2020
medline:
8
7
2021
entrez:
16
7
2020
Statut:
ppublish
Résumé
Recently, liquid-liquid phase separation (LLPS) has attracted considerable attention among researchers in the life sciences as a plausible mechanism for the generation of microstructures inside cells. LLPS occurs through multiple nonspecific interactions and does not always require a lock-and-key interaction with a binary macromolecular solution. The remarkable features of LLPS include the non-uniform localization and concentration of solutes, resulting in the ability to isolate certain chemical systems and thereby parallelize multiple chemical reactions within the limited space of a living cell. We report that, by using the macromolecules, poly(ethylene glycol) (PEG) and dextran, that exhibit LLPS in an aqueous solution, cell-sized liposomes are spontaneously formed therein in the presence of phospholipids. In this system, LLPS is generated through the depletion effect of macromolecules. The results showed that cell-like microdroplets entrapping DNA wrapped by a phospholipid layer emerge in a self-organized manner.
Identifiants
pubmed: 32667694
doi: 10.1002/cbic.202000344
pmc: PMC7754443
doi:
Substances chimiques
Dextrans
0
Macromolecular Substances
0
Phospholipids
0
Solutions
0
Water
059QF0KO0R
Polyethylene Glycols
3WJQ0SDW1A
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3323-3328Informations de copyright
© 2020 The Authors. Published by Wiley-VCH GmbH.
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