Curcumin preconditioned human adipose derived stem cells co-transplanted with platelet rich plasma improve wound healing in diabetic rats.
Adipose Tissue
/ cytology
Animals
Cell Movement
/ physiology
Cell Proliferation
/ physiology
Combined Modality Therapy
Curcumin
/ pharmacology
Diabetes Mellitus, Experimental
/ complications
Female
Glucose
/ metabolism
Humans
Platelet-Rich Plasma
Rats
Rats, Wistar
Stem Cell Transplantation
/ methods
Wound Healing
/ physiology
Curcumin
Diabetic wounds
Human adipose derived stem cells
Platelet-rich plasma
Preconditioning
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Sep 2020
15 Sep 2020
Historique:
received:
04
03
2020
revised:
23
06
2020
accepted:
09
07
2020
pubmed:
16
7
2020
medline:
18
9
2020
entrez:
16
7
2020
Statut:
ppublish
Résumé
Inflammatory and oxidative microenvironment at diabetic' wound site hinder the therapeutic efficacy of cell-based therapies in diabetic patients. The purpose of this study is to explore the competence of curcumin preconditioned human adipose derived cells (hASCs) in combination with platelet rich plasma (PRP) for the repair of wounds in diabetic rats. The cytoprotective effect of curcumin preconditioning for hASCs against hyperglycemic stress was evaluated through analysis of cell morphology, viability, cytotoxicity, senescence, and scratch wound healing assays. Subsequently, the healing capacity of curcumin preconditioned hASCs (Cur-hASCs) added to PRP was examined in excisional wounded diabetic rat model. Healed skin biopsies were excised to analyze gene and protein expression of wound healing markers by qPCR and western blotting. Histopathological changes were observed through hematoxylin and eosin staining. We found that Cur-hASCs counteract the glucose stress much better than non-preconditioned hASCs by maintaining their cellular morphology and viability as well as metabolic potential. Further in vivo results revealed that, Cur-hASCs co-injected with PRP resulted in faster wound closure, improved fibroblast proliferation, increased neovascularization, marked reduction in inflammatory cells, and compact extracellular matrix with completely covered thick epithelium. Moreover, Cur-hASCs + PRP treatment significantly improved the expression of key healing markers such as pro-angiogenic (Vegf), dermal matrix deposition (Col1α1), cell migration (bFgf) and cell proliferation (Pcna) at wound site. Our findings propose a combinatorial therapy (Cur-hASCs + PRP) as a novel modality to improve the efficacy of hASCs-based therapy for diabetic wounds.
Identifiants
pubmed: 32668325
pii: S0024-3205(20)30842-0
doi: 10.1016/j.lfs.2020.118091
pii:
doi:
Substances chimiques
Curcumin
IT942ZTH98
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118091Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest All authors declared no conflict of interest.