Direct on-the-spot detection of SARS-CoV-2 in patients.
Betacoronavirus
/ isolation & purification
COVID-19
Coronavirus Infections
/ diagnosis
Humans
Mass Screening
/ methods
Molecular Diagnostic Techniques
/ methods
Nucleic Acid Amplification Techniques
/ methods
Pandemics
Pneumonia, Viral
/ diagnosis
SARS-CoV-2
Saliva
/ virology
Sensitivity and Specificity
Covid-19
Molecular
RT-LAMP
SARS-CoV-2
pandemic
surveillance
Journal
Experimental biology and medicine (Maywood, N.J.)
ISSN: 1535-3699
Titre abrégé: Exp Biol Med (Maywood)
Pays: England
ID NLM: 100973463
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
pubmed:
17
7
2020
medline:
25
8
2020
entrez:
17
7
2020
Statut:
ppublish
Résumé
Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.
Identifiants
pubmed: 32668983
doi: 10.1177/1535370220941819
pmc: PMC7385438
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1187-1193Références
J Virol Methods. 2016 Dec;238:86-93
pubmed: 27793644
Clin Chem. 2020 Jul 1;66(7):975-977
pubmed: 32315390
N Engl J Med. 2020 May 21;382(21):2063
pubmed: 32294374
Biotechniques. 2015 Feb 01;58(2):59-68
pubmed: 25652028
J Clin Microbiol. 2020 May 26;58(6):
pubmed: 32245835
J Virol Methods. 2018 May;255:91-97
pubmed: 29474813
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
Clin Infect Dis. 2020 Dec 31;71(11):2939-2946
pubmed: 32562544