Metabolic engineering of phosphite metabolism in
Biological contamination
Outdoor cultivation
Phosphite metabolism
Phosphite oxidoreductase
Selectable marker
Synechococcus elongatus PCC 7942
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
05
2020
accepted:
02
07
2020
entrez:
17
7
2020
pubmed:
17
7
2020
medline:
17
7
2020
Statut:
epublish
Résumé
The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes. Here, we describe the implementation in the cyanobacterium Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data show that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium
Sections du résumé
BACKGROUND
BACKGROUND
The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes.
RESULTS
RESULTS
Here, we describe the implementation in the cyanobacterium
CONCLUSIONS
CONCLUSIONS
Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data show that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium
Identifiants
pubmed: 32670406
doi: 10.1186/s13068-020-01759-z
pii: 1759
pmc: PMC7346359
doi:
Types de publication
Journal Article
Langues
eng
Pagination
119Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsAuthors declare that at the time of doing research reported in this paper, D.L.L.-A., N.P.-G. and P.E.-H. were employees at StelaGenomics. D.L.L.-A. and L.H.-E. are stock owners of StelaGenomics.
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