Involvement of interleukins-17 and -34 in exacerbated orthodontic root resorption by jiggling force during rat experimental tooth movement.
Animals
Body Weight
Dental Cementum
/ metabolism
Immunohistochemistry
/ methods
Interleukin-17
/ metabolism
Interleukins
/ metabolism
Male
Osteoclasts
/ metabolism
Osteogenesis
Periodontal Ligament
/ pathology
Rats
Rats, Wistar
Root Resorption
/ etiology
T-Lymphocytes
/ metabolism
Tartrate-Resistant Acid Phosphatase
Tooth Movement Techniques
/ adverse effects
IL-17
IL-34
Jiggling force
Orthodontic force
Root resorption
Tooth movement
Journal
Journal of the World federation of orthodontists
ISSN: 2212-4438
Titre abrégé: J World Fed Orthod
Pays: United States
ID NLM: 101592569
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
12
02
2020
revised:
24
04
2020
accepted:
27
04
2020
entrez:
17
7
2020
pubmed:
17
7
2020
medline:
12
10
2021
Statut:
ppublish
Résumé
Orthodontically induced root resorption (OIRR) is considered as an undesirable and unpredictable sequel of orthodontic treatment. Recent reports demonstrated that interleukin (IL)-17/IL-34, and T cells secrete inflammatory/osteoclastogenic cytokines, which might stimulate osteoclastogenesis/bone resorption. However, little is known about the role played by IL-17/IL-34 in OIRR. The present study was aimed at investigating the odontoclastic expression pattern of IL-17 and IL-34 in resorbed cementum during different experimental tooth movements in vivo. Twenty-four 8-week-old male Wistar rats were divided into four groups: control group, optimal force group (10 g), heavy force group (50 g), and jiggling force group (compression and tension, repetition; 10 g). After 7, 14, and 21 days, the expression levels of IL-17 and IL-34 protein in the resorbed cementum were analyzed using immunohistochemical methods. On day 21, the immunoreactivity for IL-17 and IL-34 in resorbed roots in the jiggling force group was stronger than that in the heavy force and optimal force groups. Moreover, the number of IL-17-positive and IL-34-positive odontoclasts was significantly increased in the jiggling force group compared with those in the other groups on day 21. These results suggest that jiggling forces might exacerbate OIRR compared with heavy forces, as evidenced by the increased expression of IL-17 and IL-34 in odontoclasts obtained from resorbed roots.
Sections du résumé
BACKGROUND
Orthodontically induced root resorption (OIRR) is considered as an undesirable and unpredictable sequel of orthodontic treatment. Recent reports demonstrated that interleukin (IL)-17/IL-34, and T cells secrete inflammatory/osteoclastogenic cytokines, which might stimulate osteoclastogenesis/bone resorption. However, little is known about the role played by IL-17/IL-34 in OIRR. The present study was aimed at investigating the odontoclastic expression pattern of IL-17 and IL-34 in resorbed cementum during different experimental tooth movements in vivo.
METHODS
Twenty-four 8-week-old male Wistar rats were divided into four groups: control group, optimal force group (10 g), heavy force group (50 g), and jiggling force group (compression and tension, repetition; 10 g). After 7, 14, and 21 days, the expression levels of IL-17 and IL-34 protein in the resorbed cementum were analyzed using immunohistochemical methods.
RESULTS
On day 21, the immunoreactivity for IL-17 and IL-34 in resorbed roots in the jiggling force group was stronger than that in the heavy force and optimal force groups. Moreover, the number of IL-17-positive and IL-34-positive odontoclasts was significantly increased in the jiggling force group compared with those in the other groups on day 21.
CONCLUSIONS
These results suggest that jiggling forces might exacerbate OIRR compared with heavy forces, as evidenced by the increased expression of IL-17 and IL-34 in odontoclasts obtained from resorbed roots.
Identifiants
pubmed: 32672655
pii: S2212-4438(20)30019-9
doi: 10.1016/j.ejwf.2020.04.002
pii:
doi:
Substances chimiques
Il17a protein, rat
0
Interleukin-17
0
Interleukins
0
interleukin-34, rat
0
Tartrate-Resistant Acid Phosphatase
EC 3.1.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
47-55Informations de copyright
Copyright © 2020 World Federation of Orthodontists. Published by Elsevier Inc. All rights reserved.