Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach.
Cryo–EM
extracellular vesicles
helminth
proteomics
vesicle labelling
vesicle tracking
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
14 Jul 2020
14 Jul 2020
Historique:
received:
18
06
2020
revised:
09
07
2020
accepted:
10
07
2020
entrez:
18
7
2020
pubmed:
18
7
2020
medline:
18
7
2020
Statut:
epublish
Résumé
In the last two decades, extracellular vesicles (EVs) from the three domains of life, Archaea, Bacteria and Eukaryotes, have gained increasing scientific attention. As such, the role of EVs in host-pathogen communication and immune modulation are being intensely investigated. Pivotal to EV research is the determination of how and where EVs are taken up by recipient cells and organs in vivo, which requires suitable tracking strategies including labelling. Labelling of EVs is often performed post-isolation which increases risks of non-specific labelling and the introduction of labelling artefacts. Here we exploited the inability of helminths to de novo synthesise fatty acids to enable labelling of EVs by whole organism uptake of fluorescent lipid analogues and the subsequent incorporation in EVs. We showed uptake of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-Rho) in
Identifiants
pubmed: 32674418
pii: biomedicines8070213
doi: 10.3390/biomedicines8070213
pmc: PMC7399896
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Danmarks Frie Forskningsfond
ID : DFF-6111-00521
Organisme : Danish National Mass Spectrometry Platform for Functional Proteomics
ID : 5072-00007B
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