Dried Urine Microsampling Coupled to Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the Analysis of Unconjugated Anabolic Androgenic Steroids.
LC-MS/MS
anabolic androgenic steroids
anti-doping
dried urine spots (DUS)
microsampling
neuroprotection
volumetric absorptive microsampling (VAMS)
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
14 Jul 2020
14 Jul 2020
Historique:
received:
12
06
2020
revised:
09
07
2020
accepted:
10
07
2020
entrez:
18
7
2020
pubmed:
18
7
2020
medline:
3
3
2021
Statut:
epublish
Résumé
Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation ≤1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.
Identifiants
pubmed: 32674492
pii: molecules25143210
doi: 10.3390/molecules25143210
pmc: PMC7397045
pii:
doi:
Substances chimiques
Testosterone Congeners
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Commission for the Monitoring and Control of Doping and the Protection of Health in Sporting Activities (Italian Ministry of Health)
ID : J52I14001600005
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