Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa.
allergy
commensal bacteria
host microbiome
in vitro
innate immune response
metals
oral mucosa
reconstructed human gingiva
reconstructed human skin
skin
Journal
Contact dermatitis
ISSN: 1600-0536
Titre abrégé: Contact Dermatitis
Pays: England
ID NLM: 7604950
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
10
04
2020
revised:
10
07
2020
accepted:
15
07
2020
pubmed:
18
7
2020
medline:
6
7
2021
entrez:
18
7
2020
Statut:
ppublish
Résumé
Skin and oral mucosa are continuously exposed to potential metal sensitizers while hosting abundant microbes, which may influence the host response to sensitizers. This host response may also be influenced by the route of exposure that is skin or oral mucosa, due to their different immune properties. Determine how commensal Streptococcus mitis influences the host response to nickel sulfate (sensitizer) and titanium(IV) bis(ammonium lactato)dihydroxide (questionable sensitizer) in reconstructed human skin (RHS) and gingiva (RHG). RHS/RHG was exposed to nickel or titanium, in the presence or absence of S. mitis for 24 hours. Histology, cytokine secretion, and Toll-like receptors (TLRs) expression were assessed. S. mitis increased interleukin (IL)-6, CXCL8, CCL2, CCL5, and CCL20 secretion in RHS but not in RHG; co-application with nickel further increased cytokine secretion. In contrast, titanium suppressed S. mitis-induced cytokine secretion in RHS and had no influence on RHG. S. mitis and metals differentially regulated TLR1 and TLR4 in RHS, and predominantly TLR4 in RHG. Co-exposure of S. mitis and nickel resulted in a more potent innate immune response in RHS than in RHG, whereas titanium remained inert. These results indicate the important influence of commensal microbes and the route of exposure on the host's response to metals.
Sections du résumé
BACKGROUND
BACKGROUND
Skin and oral mucosa are continuously exposed to potential metal sensitizers while hosting abundant microbes, which may influence the host response to sensitizers. This host response may also be influenced by the route of exposure that is skin or oral mucosa, due to their different immune properties.
OBJECTIVE
OBJECTIVE
Determine how commensal Streptococcus mitis influences the host response to nickel sulfate (sensitizer) and titanium(IV) bis(ammonium lactato)dihydroxide (questionable sensitizer) in reconstructed human skin (RHS) and gingiva (RHG).
METHODS
METHODS
RHS/RHG was exposed to nickel or titanium, in the presence or absence of S. mitis for 24 hours. Histology, cytokine secretion, and Toll-like receptors (TLRs) expression were assessed.
RESULTS
RESULTS
S. mitis increased interleukin (IL)-6, CXCL8, CCL2, CCL5, and CCL20 secretion in RHS but not in RHG; co-application with nickel further increased cytokine secretion. In contrast, titanium suppressed S. mitis-induced cytokine secretion in RHS and had no influence on RHG. S. mitis and metals differentially regulated TLR1 and TLR4 in RHS, and predominantly TLR4 in RHG.
CONCLUSION
CONCLUSIONS
Co-exposure of S. mitis and nickel resulted in a more potent innate immune response in RHS than in RHG, whereas titanium remained inert. These results indicate the important influence of commensal microbes and the route of exposure on the host's response to metals.
Identifiants
pubmed: 32677222
doi: 10.1111/cod.13668
pmc: PMC7693211
doi:
Substances chimiques
nickel sulfate
4FLT4T3WUN
Nickel
7OV03QG267
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
347-360Subventions
Organisme : China Fellowship Council
Informations de copyright
© 2020 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd.
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