Engineering REST-Specific Synthetic PUF Proteins to Control Neuronal Gene Expression: A Combined Experimental and Computational Study.
RNA binding proteins (RBPs)
computational modeling
free energy calculations
gene transcription
molecular dynamics simulation
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
21 08 2020
21 08 2020
Historique:
pubmed:
18
7
2020
medline:
28
5
2021
entrez:
18
7
2020
Statut:
ppublish
Résumé
Regulation of gene transcription is an essential mechanism for differentiation and adaptation of organisms. A key actor in this regulation process is the repressor element 1 (RE1)-silencing transcription factor (REST), a transcriptional repressor that controls more than 2000 putative target genes, most of which are neuron-specific. With the purpose of modulating REST expression, we exploited synthetic,
Identifiants
pubmed: 32678979
doi: 10.1021/acssynbio.0c00119
doi:
Substances chimiques
PUM1 protein, human
0
RE1-silencing transcription factor
0
RNA, Messenger
0
RNA-Binding Proteins
0
Repressor Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM