Non-retroviral Endogenous Viral Element Limits Cognate Virus Replication in Aedes aegypti Ovaries.
Aedes aegypti
CRISPR-Cas9
RNA interference
cell-fusing agent virus
endogenous viral element
insect immunity
insect-specific flavivirus
mosquito
piRNA pathway
siRNA pathway
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
21 09 2020
21 09 2020
Historique:
received:
02
04
2020
revised:
06
06
2020
accepted:
16
06
2020
pubmed:
18
7
2020
medline:
10
8
2021
entrez:
18
7
2020
Statut:
ppublish
Résumé
Endogenous viral elements (EVEs) are viral sequences integrated in host genomes. A large number of non-retroviral EVEs was recently detected in Aedes mosquito genomes, leading to the hypothesis that mosquito EVEs may control exogenous infections by closely related viruses. Here, we experimentally investigated the role of an EVE naturally found in Aedes aegypti populations and derived from the widespread insect-specific virus, cell-fusing agent virus (CFAV). Using CRISPR-Cas9 genome editing, we created an Ae. aegypti line lacking the CFAV EVE. Absence of the EVE resulted in increased CFAV replication in ovaries, possibly modulating vertical transmission of the virus. Viral replication was controlled by targeting of viral RNA by EVE-derived P-element-induced wimpy testis-interacting RNAs (piRNAs). Our results provide evidence that antiviral piRNAs are produced in the presence of a naturally occurring EVE and its cognate virus, demonstrating a functional link between non-retroviral EVEs and antiviral immunity in a natural insect-virus interaction.
Identifiants
pubmed: 32679098
pii: S0960-9822(20)30909-X
doi: 10.1016/j.cub.2020.06.057
pmc: PMC7522710
pii:
doi:
Substances chimiques
RNA, Small Interfering
0
RNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3495-3506.e6Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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