Isolation and characterization of the major centipede allergen Sco m 5 from Scolopendra subspinipes mutilans.

Allergens / chemistry Amino Acid Sequence Animals Chilopoda / immunology Chromatography, Ion Exchange Cross Reactions / immunology Enzyme-Linked Immunosorbent Assay Hypersensitivity / diagnosis Immunoglobulin E / immunology Mast Cells / immunology Skin Tests Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Allergen Centipede Cross-reactivity IgE Venom

Journal

Allergology international : official journal of the Japanese Society of Allergology
ISSN: 1440-1592
Titre abrégé: Allergol Int
Pays: England
ID NLM: 9616296

Informations de publication

Date de publication:
Jan 2021
Historique:
received: 23 06 2019
revised: 02 06 2020
accepted: 05 06 2020
pubmed: 19 7 2020
medline: 4 9 2021
entrez: 19 7 2020
Statut: ppublish

Résumé

Allergic reactions have been observed following both direct centipede bites and the clinical use of centipede-containing medicines, such as traditional Chinese medicines utilizing Scolopendra subspinipes mutilans; however, no natural centipede allergen has yet been characterized. An allergen was purified from S. s. mutilans venom using Superdex 75 gel filtration and RESOURCE S ion chromatography, and its primary structure was determined via a combination of LC-MS-MS, MALDI-TOF/TOF and protein sequencing techniques. Its potential allergenicity was evaluated by immunoblotting, ELISAs, skin prick tests (SPTs) and mast cell activation assays. A novel allergen Sco m 5 (210 amino acids long) was successfully purified from crude S. s. mutilans venom. Sco m 5 could promote the degranulation of a human mast cell line, HMC-1. Among centipede-allergic patients, Sco m 5 showed an 83.3% IgE-binding frequency and a 66.7% positive reaction frequency, as detected by immunoblotting and SPTs, respectively. Sco m 5 IgE-binding frequencies of common Chinese population was found to be 9%-16%. Sera positive for Sco m 5 IgE-binding was cross-reactive against venom from the wasp Vespa mandaeinia. The present study isolated and characterized a novel allergen termed as Sco m 5 from the centipede S. s. mutilans. The use of Sco m 5 to identify centipede-allergic individuals could be important, given the high potential allergenicity of Sco m 5 among the general Chinese population, along with the likely possibility of cross-reactivity against wasp venom among centipede-allergic patients.

Sections du résumé

BACKGROUND BACKGROUND
Allergic reactions have been observed following both direct centipede bites and the clinical use of centipede-containing medicines, such as traditional Chinese medicines utilizing Scolopendra subspinipes mutilans; however, no natural centipede allergen has yet been characterized.
METHODS METHODS
An allergen was purified from S. s. mutilans venom using Superdex 75 gel filtration and RESOURCE S ion chromatography, and its primary structure was determined via a combination of LC-MS-MS, MALDI-TOF/TOF and protein sequencing techniques. Its potential allergenicity was evaluated by immunoblotting, ELISAs, skin prick tests (SPTs) and mast cell activation assays.
RESULTS RESULTS
A novel allergen Sco m 5 (210 amino acids long) was successfully purified from crude S. s. mutilans venom. Sco m 5 could promote the degranulation of a human mast cell line, HMC-1. Among centipede-allergic patients, Sco m 5 showed an 83.3% IgE-binding frequency and a 66.7% positive reaction frequency, as detected by immunoblotting and SPTs, respectively. Sco m 5 IgE-binding frequencies of common Chinese population was found to be 9%-16%. Sera positive for Sco m 5 IgE-binding was cross-reactive against venom from the wasp Vespa mandaeinia.
CONCLUSIONS CONCLUSIONS
The present study isolated and characterized a novel allergen termed as Sco m 5 from the centipede S. s. mutilans. The use of Sco m 5 to identify centipede-allergic individuals could be important, given the high potential allergenicity of Sco m 5 among the general Chinese population, along with the likely possibility of cross-reactivity against wasp venom among centipede-allergic patients.

Identifiants

DOI: 10.1016/j.alit.2020.06.003 PMID: 32680616
pubmed: 32680616
pii: S1323-8930(20)30092-7
doi: 10.1016/j.alit.2020.06.003
pii:
doi:

Substances chimiques

Allergens 0
Immunoglobulin E 37341-29-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121-128

Informations de copyright

Copyright © 2020 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.

Auteurs

Xin-Qiang Lan (XQ)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China; Key Laboratory of Ethic Medical Resources Research and Southeast Asian International Cooperation of Yunnan Universities, Department of Biology and Chemistry, Puer University, Puer, Yunnan, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China.

Feng Zhao (F)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China; Key Laboratory of Ethic Medical Resources Research and Southeast Asian International Cooperation of Yunnan Universities, Department of Biology and Chemistry, Puer University, Puer, Yunnan, China.

Qi-Quan Wang (QQ)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China.

Jiang-Hua Li (JH)

Puer People's Hospital, Puer, Yunnan, China.

Lin Zeng (L)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China.

Yun Zhang (Y)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China; CAS Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, China. Electronic address: zhangy@mail.kiz.ac.cn.

Wen-Hui Lee (WH)

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China. Electronic address: leewh@mail.kiz.ac.cn.

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Classifications MeSH

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questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Isolation and characterization of the major...