Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium.
anaerobic consortium
lignocellulose degradation
metagenome
metasecretome
polysaccharide utilization loci
rumen
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
25
01
2020
accepted:
10
07
2020
pubmed:
19
7
2020
medline:
15
12
2020
entrez:
19
7
2020
Statut:
epublish
Résumé
Lignocellulose is one of the most abundant renewable carbon sources, representing an alternative to petroleum for the production of fuel and chemicals. Nonetheless, the lignocellulose saccharification process, to release sugars for downstream applications, is one of the most crucial factors economically challenging to its use. The synergism required among the various carbohydrate-active enzymes (CAZymes) for efficient lignocellulose breakdown is often not satisfactorily achieved with an enzyme mixture from a single strain. To overcome this challenge, enrichment strategies can be applied to develop microbial communities with an efficient CAZyme arsenal, incorporating complementary and synergistic properties, to improve lignocellulose deconstruction. We report a comprehensive and deep analysis of an enriched rumen anaerobic consortium (ERAC) established on sugarcane bagasse (SB). The lignocellulolytic abilities of the ERAC were confirmed by analyzing the depolymerization of bagasse by scanning electron microscopy, enzymatic assays, and mass spectrometry. Taxonomic analysis based on 16S rRNA sequencing elucidated the community enrichment process, which was marked by a higher abundance of
Identifiants
pubmed: 32680862
pii: AEM.00199-20
doi: 10.1128/AEM.00199-20
pmc: PMC7480376
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Polysaccharides
0
lignocellulose
11132-73-3
Cellulose
9004-34-6
Lignin
9005-53-2
bagasse
9006-97-7
Cellulases
EC 3.2.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Tomazetto et al.
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