An antibody panel for highly specific detection and differentiation of Zika virus.
Animals
Antibodies, Viral
/ immunology
Antibody Specificity
/ immunology
Chlorocebus aethiops
Cross Reactions
/ immunology
Dengue Virus
/ immunology
Limit of Detection
Mice
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Vero Cells
Viral Envelope Proteins
/ immunology
Zika Virus
/ immunology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 07 2020
17 07 2020
Historique:
received:
19
11
2019
accepted:
29
06
2020
entrez:
19
7
2020
pubmed:
19
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. We aimed to assemble a highly specific anti-Zika antibody panel to be utilized in the development of a highly specific and cost-effective ZIKV rapid quantification assay for viral load monitoring at point-of-care settings. To this end, we tested the affinity and specificity of twenty one commercially available monoclonal and polyclonal antibodies against ZIKV and DENV envelope proteins utilizing nine ZIKV and twelve DENV strains. We finalized and tested a panel of five antibodies for the specific detection and differentiation of ZIKV and DENV infected samples.
Identifiants
pubmed: 32681135
doi: 10.1038/s41598-020-68635-6
pii: 10.1038/s41598-020-68635-6
pmc: PMC7367842
doi:
Substances chimiques
Antibodies, Viral
0
Viral Envelope Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11906Subventions
Organisme : NIAID NIH HHS
ID : R15 AI127214
Pays : United States
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