Analysis of RNA-DNA Triplex Structures In Vitro and In Vivo.
Biotinylated RNA
Electrophoretic mobility shift assay
Noncoding RNA
Psoralen
Pull-down
RNA–DNA triplexes
Triplex capture
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
19
7
2020
pubmed:
19
7
2020
medline:
13
3
2021
Statut:
ppublish
Résumé
RNA can bind within the major groove of purine-rich DNA via Hoogsteen base pairing and form a triple helical RNA-DNA structure that anchors the RNA to specific DNA sequences, thereby targeting RNA-associated regulatory proteins to distinct genomic sites. Here we present methods to analyze the potential of a given RNA to form triplexes in vitro and to validate these structures in vivo.
Identifiants
pubmed: 32681516
doi: 10.1007/978-1-0716-0680-3_16
doi:
Substances chimiques
triplex DNA
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM