The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche.
Azoospermia
Human Serum Albumin
Scaffold
Spermatogenesis
Testis
Journal
International journal of fertility & sterility
ISSN: 2008-076X
Titre abrégé: Int J Fertil Steril
Pays: Iran
ID NLM: 101487941
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
05
09
2019
accepted:
24
11
2019
entrez:
19
7
2020
pubmed:
19
7
2020
medline:
19
7
2020
Statut:
ppublish
Résumé
Azoospermia is one of the challenging disorders affecting couples who are afflicted with infertility. Human testisderived cells (hTCs) are suitable candidates for the initiation of in-vitro spermatogenesis for these types of patients. The current study aimed to assess the proliferation of hTCs through the cell culture on the three-dimensional (3D) porous scaffolds. Cells harvested from the testicular sperm extraction (TESE) samples of the azoospermic patients were cultured on the 3D porous scaffolds containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) for two weeks. The proliferation/viability of the cells was assessed using the MTT assay, along with H and E histological staining method. The MTT assay showed that hTCs could stay alive on this scaffold with 50 and 66.66% viability after 7 and 14 days, respectively. Such viability was not significantly different when compared with cells grown on monolayer flask culture (P>0.05). Therefore, 3D HSA/TCP NPs scaffolds could be used for the reconstitution of the artificial human somatic testicular niche for future applications in regenerative medicine for male infertility.
Identifiants
pubmed: 32681628
doi: 10.22074/ijfs.2020.6086
pmc: PMC7382676
doi:
Types de publication
Journal Article
Langues
eng
Pagination
150-153Informations de copyright
Copyright© by Royan Institute. All rights reserved.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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