A humanized antibody inhibitor for cathepsin L.
antibody
cathepsin L
protease inhibitor
protein engineering
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
26
05
2020
revised:
11
07
2020
accepted:
13
07
2020
pubmed:
20
7
2020
medline:
5
2
2021
entrez:
20
7
2020
Statut:
ppublish
Résumé
Cathepsin L (CTSL) is a cysteine protease involved in a variety of physiological and pathological processes. Potent inhibitors against CTSL have long been sought for drug development. Due to insufficient specificity and suboptimal pharmacological properties for current CTSL inhibitors, novel agents are still required for selectively blocking CTSL activity. Here we generated a humanized antibody inhibitor of CTSL by genetically fusing the inhibitory propeptide of procathepsin L to the N-terminus of the light chain of a humanized antibody. The resulting antibody fusion could be stably expressed and displays highly potent inhibition activity and specificity toward CTSL. This work demonstrates a new approach for the rapid generation of antibody inhibitors of CTSL. It can possibly be extended to create inhibitory antibodies targeting other cathepsin proteases, providing novel research and therapeutic tools.
Identifiants
pubmed: 32683733
doi: 10.1002/pro.3913
pmc: PMC7454522
doi:
Substances chimiques
Antibodies, Monoclonal, Humanized
0
Cysteine Proteinase Inhibitors
0
CTSL protein, human
EC 3.4.22.15
Cathepsin L
EC 3.4.22.15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1924-1930Informations de copyright
© 2020 The Protein Society.
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