High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States.


Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
19 08 2020
Historique:
pubmed: 21 7 2020
medline: 16 4 2021
entrez: 21 7 2020
Statut: ppublish

Résumé

Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that catalyzes the conversion of methane to methanol at ambient temperature using a nonheme, oxygen-bridged dinuclear iron cluster in the active site. Structural changes in the hydroxylase component (sMMOH) containing the diiron cluster caused by complex formation with a regulatory component (MMOB) and by iron reduction are important for the regulation of O

Identifiants

pubmed: 32683863
doi: 10.1021/jacs.0c05613
pmc: PMC7457426
mid: NIHMS1620311
doi:

Substances chimiques

Oxygenases EC 1.13.-
methane monooxygenase EC 1.14.13.25

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

14249-14266

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : 102593
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : T32 GM008347
Pays : United States
Organisme : NIH HHS
ID : S10 OD023453
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126289
Pays : United States
Organisme : NIGMS NIH HHS
ID : R56 GM055302
Pays : United States
Organisme : Wellcome Trust
ID : 210734/Z/18/Z
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM110501
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM133081
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118030
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM055302
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM117126
Pays : United States

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Auteurs

Vivek Srinivas (V)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

Rahul Banerjee (R)

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55391, United States.

Hugo Lebrette (H)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

Jason C Jones (JC)

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55391, United States.

Oskar Aurelius (O)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

In-Sik Kim (IS)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Cindy C Pham (CC)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Sheraz Gul (S)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Kyle D Sutherlin (KD)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Asmit Bhowmick (A)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Juliane John (J)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

Esra Bozkurt (E)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

Thomas Fransson (T)

Interdisciplinary Center for Scientific Computing, University of Heidelberg, 69120 Heidelberg, Germany.

Pierre Aller (P)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom.

Agata Butryn (A)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom.

Isabel Bogacz (I)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Philipp Simon (P)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Stephen Keable (S)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Alexander Britz (A)

LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.

Kensuke Tono (K)

Japan Synchrotron Radiation Research Institute, Sayo-gun 679 5198, Japan.

Kyung Sook Kim (KS)

Pohang Accelerator Laboratory, Gyeongsangbuk-do 37673, South Korea.

Sang-Youn Park (SY)

Pohang Accelerator Laboratory, Gyeongsangbuk-do 37673, South Korea.

Sang Jae Lee (SJ)

Pohang Accelerator Laboratory, Gyeongsangbuk-do 37673, South Korea.

Jaehyun Park (J)

Pohang Accelerator Laboratory, Gyeongsangbuk-do 37673, South Korea.

Roberto Alonso-Mori (R)

LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.

Franklin D Fuller (FD)

LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.

Alexander Batyuk (A)

LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.

Aaron S Brewster (AS)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Uwe Bergmann (U)

Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.

Nicholas K Sauter (NK)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Allen M Orville (AM)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom.
Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxfordshire OX11 0FA, United Kingdom.

Vittal K Yachandra (VK)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Junko Yano (J)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

John D Lipscomb (JD)

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55391, United States.

Jan Kern (J)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Martin Högbom (M)

Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm 106 91, Sweden.

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