Direct Phosphorylation of Psilocin Enables Optimized cGMP Kilogram-Scale Manufacture of Psilocybin.


Journal

ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658

Informations de publication

Date de publication:
14 Jul 2020
Historique:
received: 21 05 2020
accepted: 17 06 2020
entrez: 21 7 2020
pubmed: 21 7 2020
medline: 21 7 2020
Statut: epublish

Résumé

A second-generation kilogram-scale synthesis of the psychedelic tryptamine psilocybin has been developed. The synthesis was designed to address several challenges first encountered with the scale-up of previously described literature procedures, which were not optimized for providing consistent yield and purity of products, atom economy, or being run in pilot plant-scale reactors. These challenges were addressed and circumvented with the design of the second-generation route, which featured an optimized cGMP large-scale Speeter-Anthony tryptamine synthesis to the intermediate psilocin with improved in-process control and impurity removal over the three steps. Psilocin was subsequently phosphorylated directly with phosphorous oxychloride for the first time, avoiding a tedious and poor atom economy benzyl-protecting group strategy common to all previously described methods for producing psilocybin. In this report, the challenges encountered in a 100 g scale first-generation literature-based synthesis are highlighted, followed by a detailed description of the newly developed second-generation synthesis to provide over one kilogram of high-purity psilocybin under cGMP.

Identifiants

pubmed: 32685866
doi: 10.1021/acsomega.0c02387
pmc: PMC7364850
doi:

Types de publication

Journal Article

Langues

eng

Pagination

16959-16966

Informations de copyright

Copyright © 2020 American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

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Auteurs

Robert B Kargbo (RB)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Alexander Sherwood (A)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Andrew Walker (A)

Almac Sciences, 20 Seagoe Industrial Estate, Craigavon BT63 5QD, United Kingdom.

Nicholas V Cozzi (NV)

Neuropharmacology Laboratory, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, United States.
Alexander Shulgin Research Institute, 1483 Shulgin Road, Lafayette, California 94549, United States.

Raymond E Dagger (RE)

Stonehedge Pharmaceutical Consulting, LLC, 13121 Old Annapolis Road, Mount Airy, Maryland 21771, United States.

Jessica Sable (J)

Promega Corporation, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Kelsey O'Hern (K)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Kristi Kaylo (K)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Tura Patterson (T)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Gary Tarpley (G)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Poncho Meisenheimer (P)

Usona Institute, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.

Classifications MeSH