Prognostic impact of HERC2 protein and pink-eyed dilution protein in uveal melanoma.
Adult
Biomarkers, Tumor
/ analysis
Disease-Free Survival
Female
Gene Expression
Guanine Nucleotide Exchange Factors
/ analysis
Humans
Immunohistochemistry
Male
Melanoma
/ diagnosis
Membrane Transport Proteins
/ analysis
Middle Aged
Predictive Value of Tests
Prognosis
Ubiquitin-Protein Ligases
Uveal Neoplasms
/ diagnosis
HERC2
Metastasis-free survival
P-protein
Pigmentation
Uveal melanoma
Journal
Human cell
ISSN: 1749-0774
Titre abrégé: Hum Cell
Pays: Japan
ID NLM: 8912329
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
09
05
2020
accepted:
08
07
2020
pubmed:
21
7
2020
medline:
28
10
2020
entrez:
21
7
2020
Statut:
ppublish
Résumé
Biological understanding of pigmentation and its association with clinicopathological implications in uveal melanoma (UM) risk is still unexplored. HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase 2 (HERC2) and Pink-eyed dilution protein (P-protein) are the important markers that regulate pigmentation in eye. Therefore, our aim of the study was to investigate the expression of HERC2 and P-protein in the UM patients and correlate with patient outcome. Fifty-two formalin-fixed paraffin-embedded UM tissue samples were included to detect the expression of HERC2 and P-protein by immunohistochemistry and validated by western blot. Cox proportional hazard model and log-rank test were used to determine the prognostic potential of these proteins. High pigmentation was seen in 67% of the UM cases. The expression of HERC2 and P-protein was present in 44% and 71% cases, respectively. On statistical analysis, increased pigmentation, epithelioid cell type, and ciliary body invasion were significant with the protein expressions (p < 0.05). Metastasis-free survival was reduced in UM cases which expressed HERC2 and P-protein. On multivariate analysis, P-protein expression was found to be an independent prognostic factor. Our findings suggest that HERC2 and P-protein could be used as novel predictors of high pigmentation in UM cases which have high metastatic potential.
Identifiants
pubmed: 32686068
doi: 10.1007/s13577-020-00397-9
pii: 10.1007/s13577-020-00397-9
doi:
Substances chimiques
Biomarkers, Tumor
0
Guanine Nucleotide Exchange Factors
0
Membrane Transport Proteins
0
OCA2 protein, human
0
HERC2 protein, human
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1264-1272Subventions
Organisme : All-India Institute of Medical Sciences
ID : Intramural research grant (IRG)
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