Biomanagement of rice root-knot nematode Meloidogyne graminicola using five indigenous microbial isolates under pot and field trials.
biocontrol bacteria
biocontrol fungi
microbial antagonism
root-dip treatment
soil application
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
01
02
2020
revised:
13
07
2020
accepted:
13
07
2020
pubmed:
21
7
2020
medline:
20
2
2021
entrez:
21
7
2020
Statut:
ppublish
Résumé
To ascertain the effectiveness of Aspergillus niger, Trichoderma harzianum, Pochonia chlamydosporia, Bacillus subtilis and Pseudomonas fluorescens against rice root-knot nematode, Meloidogyne graminicola, and to optimize their application methods. The relative effectiveness of five indigenous biocontrol agents (BCA) against M. graminicola on rice cv. PS-5 was tested initially in pot culture. The BCAs, A. niger, P. chlamydosporia and P. fluorescens proved more effective, and significantly reduced the nematode disease. It is hypothesized that success of a biocontrol module may vary with the BCA and application methods. Hence, the effectiveness of the above three BCAs as well as seven different treatment schemes were evaluated in naturally infested farmer's fields during 2 consecutive years. In nematode-infested plots without any BCA treatments, terminal galls formed on the roots, and plants suffered a 19-31% decrease in the growth and yield. The treatments with P. chlamydosporia or A. niger through root-dip (RD) plus one soil application (SA) at 15 days after planting were found to be highly effective against the nematode. Relatively greater nematode control was achieved with RD plus two SAs (15 + 30 DAP) but statistically the effect was on par with RD + one SA at 15 DAP. These treatments significantly reduced galling (22-25%), egg mass production (21-29%) and reproduction factor (63-70%) of M. graminicola, and subsequently increased the grain yield (11-21%). Application methods enhanced the effectiveness of BCAs against M. graminicola. The RD plus one SA at 15 DAP proved to be most effective treatment to control root-knot disease in rice. Use of multiple treatments (root dip and SA) appears cumbersome, but in view of effectiveness and limitation of chemical control in rice paddies, farmers may adopt the above module that may lead to 11-21% yield improvement.
Substances chimiques
Biological Control Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
424-438Informations de copyright
© 2020 The Society for Applied Microbiology.
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