Differences in pig respiratory tract and peripheral blood immune responses to Actinobacillus pleuropneumoniae.
Actinobacillus Infections
/ blood
Actinobacillus pleuropneumoniae
/ immunology
Animals
Bronchoalveolar Lavage Fluid
/ microbiology
Chemokines
/ immunology
Cytokines
/ immunology
Immunity
Leukocytes, Mononuclear
/ immunology
Lung
/ microbiology
Lymphocytes
/ immunology
Respiratory System
/ microbiology
Specific Pathogen-Free Organisms
Swine
Transcriptome
Actinobacillus pleuropneumoniae
Bronchoalveolar lavage fluid
Inflammatory cytokines
Peripheral blood
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
22
11
2019
revised:
25
04
2020
accepted:
11
06
2020
pubmed:
21
7
2020
medline:
8
6
2021
entrez:
21
7
2020
Statut:
ppublish
Résumé
Excessive cytokine production is an important component of the acute respiratory distress syndrome and multiple organ failure. Pneumonia can lead to an overexpression of cytokines, although comparatively little is known about the relevance and differences in cytokines between blood and lung. In this study, piglets were experimentally infected intranasally with Actinobacillus pleuropneumoniae (APP), and transcriptomes of lung tissue and peripheral blood mononuclear cells determined. In addition, the levels of 30 cytokines in broncheoalveolar lavage fluid (BALF) and sera were determined by ELISA. Post infection, there was an early increase in lung monocytes, and a later rise in inflammatory cytokines in BALF. Blood lymphocytes increased early in infection and there was a rise in inflammatory cytokines in the peripheral blood of infected piglets. Genes involved in cytokine production, leukocyte migration and differentiation, lymphocyte activation, and cytokine-mediated signaling pathways in the transcriptomes of lung tissue were significantly down-regulated early in infection. At this early phase of APP infection (0-6 h), the cytokines IL-1β, MCP-1, and IL-5 in sera increased rapidly and significantly, while many cytokines in BALF decreased. At 48 h post-infection, cytokines in sera were no longer significantly increased, although some were up-regulated in BALF, and there was aggravated pathological damage in the lungs at this time. The data indicate there are substantial differences between immune cells and cytokines in the lung and peripheral blood of APP infected piglets at equivalent time points. The results increase our understanding of pig-APP host interactive biology, and will be important in formulating future therapeutic and preventative strategies to prevent disease caused by APP.
Identifiants
pubmed: 32686648
pii: S0378-1135(19)31371-9
doi: 10.1016/j.vetmic.2020.108755
pii:
doi:
Substances chimiques
Chemokines
0
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108755Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest All authors declare that there are no conflicts of interest.