Zinc oxide nanoparticles from Cyperus rotundus attenuates diabetic retinopathy by inhibiting NLRP3 inflammasome activation in STZ-induced diabetic rats.
Animals
Cyperus
/ chemistry
Diabetes Mellitus, Experimental
/ complications
Diabetic Nephropathies
/ complications
Feeding Behavior
Glycated Hemoglobin
/ metabolism
Inflammasomes
/ drug effects
Insulin
/ blood
Male
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Nanoparticles
/ chemistry
Rats
Rats, Sprague-Dawley
Spectrum Analysis
/ methods
Streptozocin
X-Ray Diffraction
Zinc Oxide
/ chemistry
Cyperus rotundus
diabetic retinopathy
inflammasome
zinc oxide nanoparticles
Journal
Journal of biochemical and molecular toxicology
ISSN: 1099-0461
Titre abrégé: J Biochem Mol Toxicol
Pays: United States
ID NLM: 9717231
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
10
09
2019
revised:
22
06
2020
accepted:
30
06
2020
pubmed:
22
7
2020
medline:
3
7
2021
entrez:
22
7
2020
Statut:
ppublish
Résumé
Diabetic retinopathy (DRP) is a retinal disease caused by diabetes mellitus, which is categorized by microvascular lesions present in the retina such as vascular leakage, vascular proliferation, and retinal ischemia. The plan of the present study was to the synthesis of Cyperus rotundus-loaded zinc oxide nanoparticles (CR-ZnONPs) which was confirmed by the various characterization methods such as UV-vis spectroscopy, energy dispersive X-ray analysis, Fourier transform infrared, scanning electron microscope, and X-ray diffraction. Also, the effect of CR-ZnONPs on DRP-induced rats was determined by food intake, fasting blood glucose (FBG), HbA1c, insulin, retina thickness, inner nuclear layer (INL), outer nuclear layer (ONL) thickness, lipid peroxidation (LPO), catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Furthermore, the status of oxidative stress marker genes (heme oxygenase-1 [HO-1] and nuclear factor erythroid 2-related factor-2 [Nrf2]) and inflammatory marker (procaspase-1, cleaved-caspase-1, interleukin-1β [IL-1β], IL-18, and ASC) expressions were examined by using real-time polymerase chain reaction and Western blot analysis techniques. We noted that the synthesized CR-ZnONPs have a crystalline structure, spherical shape, and present various functional groups. The administration of streptozotocin (STZ)-induced DRP rats were increased in the levels of HbA1c, FBG, food intake, LPO, and reduced levels of insulin, SOD, GPx, and CAT. In addition, the gene and protein expression showed the downregulation of HO-1 and Nrf2 and upregulation of procaspase-1, IL-1β, cleaved-caspase-1, IL-18, and ASC in diabetic rats. Moreover, further histopathological analysis of retinal tissues again confirmed the results of biochemical parameters. In contrast, the DRP rats treated with CR-ZnONPs significantly brought down all the parameters to normal, which indicated that the CR-ZnONPs have better antidiabetic and anti-inflammatory properties.
Substances chimiques
Glycated Hemoglobin A
0
Inflammasomes
0
Insulin
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Nlrp3 protein, rat
0
Streptozocin
5W494URQ81
Zinc Oxide
SOI2LOH54Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e22583Informations de copyright
© 2020 Wiley Periodicals LLC.
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