The Respiratory Microbiome in Cystic Fibrosis: Compartment Patterns and Clinical Relationships in Early Stage Disease.
Staphylococcus aureus
cystic fibrosis
exacerbation
lung function
microbiome
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
17
03
2020
accepted:
04
06
2020
entrez:
23
7
2020
pubmed:
23
7
2020
medline:
23
7
2020
Statut:
epublish
Résumé
We compared the bacterial microbiomes lodged in the bronchial tree, oropharynx and nose of patients with early stage cystic fibrosis (CF) not using chronic antibiotics, determining their relationships with lung function and exacerbation frequency. CF patients were enrolled in a cohort study during stability and were checked regularly over the following 9 months. Upper respiratory samples (sputum [S], oropharyngeal swab [OP] and nasal washing [N]) were collected at the first visit and every 3 months. 16S rRNA gene amplification and sequencing was performed and analyzed with QIIME. Seventeen CF patients were enrolled (16.6 SD 9.6 years). Alpha-diversity of bacterial communities between samples was significantly higher in S than in OP (Shannon index median 4.6 [IQR: 4.1-4.9] vs. 3.7 [IQR: 3-1-4.1],
Identifiants
pubmed: 32695090
doi: 10.3389/fmicb.2020.01463
pmc: PMC7339930
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1463Informations de copyright
Copyright © 2020 Garcia-Nuñez, Garcia-Gonzalez, Pomares, Montón, Millares, Quero, Prina, Asensio, Bosque, Capilla, Cuevas and Monsó.
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