Construction and assessment of the immunogenicity and bactericidal activity of fusion protein porin A from

Adjuvant Fusion protein Neisseria meningitidis Outer membrane vesicle Porin A

Journal

Iranian journal of basic medical sciences
ISSN: 2008-3866
Titre abrégé: Iran J Basic Med Sci
Pays: Iran
ID NLM: 101517966

Informations de publication

Date de publication:
Jun 2020
Historique:
entrez: 23 7 2020
pubmed: 23 7 2020
medline: 23 7 2020
Statut: ppublish

Résumé

The porins A and B and also outer membrane vesicles (OMVs) of After bioinformatic studies, a fusion protein composed of porin A from both serogroups A and B of Mice received the PorA protein plus Freund's adjuvant. Mice vaccinated with PorA fusion of serogroups A+B plus Freund's adjuvant produced more IgG, IgG1, and IgG2a than combinations admixed with OMV. Furthermore, the vaccinated mice tended to direct the IgG responses toward IgG1. Sera of the mice that received PorA+Freund's and those that received PorA+OMV produced higher bactericidal activity than the controls. Fusion protein porin A could be a valuable target for developing vaccines against

Identifiants

pubmed: 32695289
doi: 10.22038/ijbms.2020.40470.9
pmc: PMC7351441
doi:

Types de publication

Journal Article

Langues

eng

Pagination

737-743

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Auteurs

Parviz Afrough (P)

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.

Mohammad Reza Asadi Karam (MR)

Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.

Farzam Vaziri (F)

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.

Ava Behrouzi (A)

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.

Seyed Davar Siadat (SD)

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Classifications MeSH