Scribble, Lgl1, and myosin II form a complex in vivo to promote directed cell migration.
Cell Adhesion
/ physiology
Cell Communication
/ physiology
Cell Movement
/ genetics
Cell Polarity
/ genetics
Cytoskeletal Proteins
/ genetics
Cytoskeleton
/ metabolism
HEK293 Cells
Humans
Membrane Proteins
/ genetics
Myosin Type II
/ metabolism
Protein Binding
/ physiology
Protein Transport
/ physiology
Tumor Suppressor Proteins
/ genetics
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
15 09 2020
15 09 2020
Historique:
pubmed:
23
7
2020
medline:
23
6
2021
entrez:
23
7
2020
Statut:
ppublish
Résumé
Scribble (Scrib) and Lethal giant larvae 1 (Lgl1) are conserved polarity proteins that play important roles in different forms of cell polarity. The roles of Scrib and Lgl1 in apical-basal cell polarity have been studied extensively, but little is known about their roles in the cell polarity of migrating cells. Furthermore, the effect of Scrib and Lgl1 interaction on cell polarity is largely unknown. In this study, we show that Scrib, through its leucine-rich repeat domain, forms a complex in vivo with Lgl1. Scrib also forms a complex with myosin II, and Scrib, Lgl1, and myosin II colocalize at the leading edge of migrating cells. The cellular localization and the cytoskeletal association of Scrib and Lgl1 are interdependent, as depletion of either protein affects its counterpart. In addition, depletion of either Scrib or Lgl1 disrupts the cellular localization of myosin II. We show that depletion of either Scrib or Lgl1 affects cell adhesion through the inhibition of focal adhesion disassembly. Finally, we show that Scrib and Lgl1 are required for proper cell polarity of migrating cells. These results provide new insights into the mechanism regulating the cell polarity of migrating cells by Scrib, Lgl1, and myosin II.
Identifiants
pubmed: 32697665
doi: 10.1091/mbc.E19-11-0657
pmc: PMC7550706
doi:
Substances chimiques
Cytoskeletal Proteins
0
LLGL1 protein, human
0
Membrane Proteins
0
SCRIB protein, human
0
Tumor Suppressor Proteins
0
Myosin Type II
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2234-2248Références
Mol Biol Cell. 2006 Jul;17(7):2869-81
pubmed: 16611744
Science. 2000 Jul 7;289(5476):113-6
pubmed: 10884224
J Cell Biol. 2019 Mar 4;218(3):742-756
pubmed: 30598480
J Cell Sci. 1998 Aug;111 ( Pt 15):2085-95
pubmed: 9664030
Genes Dev. 2004 Aug 15;18(16):1909-25
pubmed: 15314019
Dev Dyn. 2007 Feb;236(2):364-73
pubmed: 17103414
J Cell Biol. 2011 Apr 18;193(2):381-96
pubmed: 21482721
J Cell Sci. 1999 Jun;112 ( Pt 12):1803-11
pubmed: 10341200
Bioarchitecture. 2016;6(2):29-38
pubmed: 26919260
Curr Biol. 2005 Feb 8;15(3):276-82
pubmed: 15694314
Biochemistry. 2000 Sep 19;39(37):11441-51
pubmed: 10985790
J Cell Mol Med. 2015 May;19(5):934-47
pubmed: 25683605
Cell Res. 2014 Apr;24(4):451-63
pubmed: 24513855
Curr Biol. 2004 Jun 8;14(11):987-95
pubmed: 15182672
Nat Rev Cancer. 2011 Dec 15;12(1):23-38
pubmed: 22169974
J Biol Chem. 2018 Mar 23;293(12):4519-4531
pubmed: 29378849
J Cell Biol. 1994 Dec;127(5):1361-73
pubmed: 7962095
Biochim Biophys Acta. 2008 Mar;1778(3):614-30
pubmed: 18005931
J Cell Sci. 2009 Jan 15;122(Pt 2):199-206
pubmed: 19118212
Cell. 2008 Nov 28;135(5):865-78
pubmed: 19041750
J Biol Chem. 2004 Jan 23;279(4):2800-8
pubmed: 14594953
Cancer Res. 2006 May 1;66(9):4725-33
pubmed: 16651425
Biochemistry. 2005 May 10;44(18):6867-76
pubmed: 15865432
Mol Biol Cell. 2012 Feb;23(4):591-601
pubmed: 22219375
Oncogene. 2007 Apr 5;26(16):2272-82
pubmed: 17043654
J Mol Biol. 2018 Sep 28;430(19):3521-3544
pubmed: 29289568
Cancer Res. 2014 Jun 1;74(11):3180-94
pubmed: 24662921
J Cell Biol. 2012 Nov 12;199(4):669-83
pubmed: 23128239
J Cell Sci. 2005 Jun 15;118(Pt 12):2579-87
pubmed: 15928049
Cell. 2001 Aug 24;106(4):489-98
pubmed: 11525734
J Cell Biochem. 2006 Oct 1;99(2):647-64
pubmed: 16791850
Hum Mol Genet. 2008 Nov 15;17(22):3552-65
pubmed: 18716323
Curr Biol. 2002 Apr 2;12(7):531-9
pubmed: 11937021
Nat Rev Mol Cell Biol. 2009 Nov;10(11):778-90
pubmed: 19851336
J Mol Biol. 2018 Sep 28;430(19):3545-3564
pubmed: 29782831
Semin Cell Dev Biol. 2008 Jun;19(3):234-44
pubmed: 18299239
Cell. 2005 May 6;121(3):451-63
pubmed: 15882626
Nature. 2000 Feb 10;403(6770):676-80
pubmed: 10688207
J Cell Biol. 2015 Oct 26;211(2):273-86
pubmed: 26483556
Essays Biochem. 2012;53:141-68
pubmed: 22928514
Oncogene. 2008 Nov 24;27(55):6888-907
pubmed: 19029932
Mol Cell Biol. 2000 Sep;20(17):6354-63
pubmed: 10938112
Nat Cell Biol. 2011 Apr;13(4):383-93
pubmed: 21423176
J Clin Invest. 2011 Nov;121(11):4257-67
pubmed: 21965329
J Cell Biol. 2006 Feb 27;172(5):759-69
pubmed: 16492808
Nat Rev Mol Cell Biol. 2010 Sep;11(9):633-43
pubmed: 20729930
Science. 2003 Dec 5;302(5651):1704-9
pubmed: 14657486
Oncogene. 2006 Jan 5;25(1):103-10
pubmed: 16170365
Dev Cell. 2015 Oct 26;35(2):199-210
pubmed: 26481050
Genes Dev. 2004 Mar 1;18(5):559-71
pubmed: 15037549
J Cell Sci. 2008 Jan 1;121(Pt 1):11-8
pubmed: 18096687
Oncogene. 2005 Apr 28;24(19):3100-9
pubmed: 15735678
Circ Res. 1991 Aug;69(2):530-9
pubmed: 1860190
J Cell Biol. 2008 Nov 3;183(3):543-54
pubmed: 18955554
J Cell Biol. 2007 Feb 26;176(5):573-80
pubmed: 17312025
J Mol Biol. 2018 Sep 28;430(19):3585-3612
pubmed: 29409995
Mol Biol Cell. 2006 Mar;17(3):1364-74
pubmed: 16394101
Trends Cell Biol. 2006 Dec;16(12):622-30
pubmed: 17067797
Nat Cell Biol. 2003 Jan;5(1):46-52
pubmed: 12510193
MethodsX. 2014 Jul 07;1:56-9
pubmed: 26150935
Nat Cell Biol. 2003 Jan;5(1):53-8
pubmed: 12510194
Cell. 2006 Jun 30;125(7):1361-74
pubmed: 16814721
J Cell Biol. 1994 Dec;127(5):1345-60
pubmed: 7962094
Sci Signal. 2009 Sep 08;2(87):ra51
pubmed: 19738201
J Biol Chem. 2009 Sep 11;284(37):24948-57
pubmed: 19553683
J Mol Biol. 2018 Sep 28;430(19):3504-3520
pubmed: 28963071
Curr Biol. 2006 Dec 19;16(24):2395-405
pubmed: 17081755
Proc Natl Acad Sci U S A. 1989 Oct;86(20):7726-30
pubmed: 2813355
J Cell Biol. 2015 Aug 17;210(4):583-94
pubmed: 26261182
J Cell Sci. 2014 Jan 15;127(Pt 2):295-304
pubmed: 24213535
Mol Biol Cell. 2004 Mar;15(3):982-9
pubmed: 14699073
Circ Res. 2013 Mar 15;112(6):924-34
pubmed: 23362312
Nat Cell Biol. 2003 Apr;5(4):301-8
pubmed: 12629547
J Cell Sci. 2013 Sep 1;126(Pt 17):3990-9
pubmed: 23813956
J Cell Sci. 2017 Jul 15;130(14):2329-2343
pubmed: 28596238
J Muscle Res Cell Motil. 1995 Aug;16(4):379-89
pubmed: 7499478